RT info:eu-repo/semantics/article T1 Enantioseparation of N-​derivatized amino acids by micro-​liquid chromatography using carbamoylated quinidine functionalized monolithic stationary phase A1 Wang, Qiqin A1 Feng, J A1 Han, H. A1 Zhu, P A1 Wu, H. A1 Marina Alegre, María Luisa A1 Crommen, Jaques A1 Jiang, Z. K1 Carbamoylated quinidine K1 Enantioseparations K1 N-derivatized amino acids K1 Monolithic columns K1 Micro-HPLC K1 Química K1 Chemistry AB In order to obtain satisfactory column permeability, efficiency and selectivity for micro-HPLC, a capillary monolithic column containing O-9-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine (MQD) as chiral selector was re-optimized. The monolithic column was used to successfully enantioresolve a wide range of N-derivatized amino acids including alanine, leucine, methionine, threonine, phenylalanine, valine, serine, isoleucine, tryptophan, and cysteine. The influence of mobile phase parameters, such as the organic solvent type and concentration, the apparent pH, and buffer concentration, on retention and enantioseparation of N-derivatized amino acids has been investigated. 3,5-dinitrobenzoyl-amino acids and 3,5-dichlorobenzoyl-amino acids were resolved into enantiomers with exceptionally high selectivity and resolution. The chemoselectivity of the monolithic column for a multicomponent mixture of N-derivatized amino acids was also investigated. A mixture of three pairs of 3,5-dichlorobenzoyl-amino acids could be fully resolved in 22.5 min. (C) 2014 Elsevier B.V. All rights reserved. SN 00219673 YR 2014 FD 2014-10-10 LK http://hdl.handle.net/10017/48267 UL http://hdl.handle.net/10017/48267 LA eng DS MINDS@UW RD 29-mar-2024