RT info:eu-repo/semantics/article
T1 A capillary micellar electrokinetic chromatography method for the stereoselective quantitation of bioallethrin in biotic and abiotic samples
A1 García González, María Ángeles
A1 Menéndez López, Nuria
A1 Boltes Espínola, Ana Karina
A1 Castro Puyana, María
A1 Marina Alegre, María Luisa
K1 Bioallethrin
K1 Capillary electrophoresis
K1 Micellar electrokinetic chromatography
K1 Cyclodextrins
K1 Pyrethroid insecticide
K1 Toxicity
K1 Non-target organism
K1 Chemistry
K1 Química
AB A capillary micellar electrokinetic chromatography (MEKC) method was developed enabling the stereoselective separation of the insecticide bioallethrin. The use of sodium deoxycholate bile salt and acetyl-beta-cyclodextrin (acetyl-beta-CD) made possible the separation of bioallethrin stereoisomers with a high enantioresolution (7.4) in a short analysis time (6.5 min). The analytical characteristics of the developed method were evaluated in terms of linearity, accuracy, precision, and limits of detection (LOD) and quantitation (LOQ) showing a good performance for the quantitation of bioallethrin stereoisomers with LODs of 0.2 and 0.3 mg/L. The developed method was applied to the stereoselective analysis of a commercial bioallethrin pediculicide formulation and to evaluate the toxicity of bioallethrin stereoisomers on the growth of the unicellular freshwater green alga Pseudokirchneriella subcapitata and on the germination of the higher plant Sorghum bicolor (non-target organisms). The analysis of the commercial pediculicide showed a good agreement between the contents determined for the two stereoisomers and those labelled in the commercial samples. Different toxic responses and biodegradation profiles were found for each stereoisomer in ecotoxicity assays. The mixture of SIR stereoisomers of bioallethrin resulted more toxic than S-bioallethrin for green algae, with EC50 values of 1.10 +/- 0.06 for the mixture and of 1.73 +/- 0.05 mg/L for the pure S-biallethrin (esbiol). Germination of plants seeds was also affected. (C) 2017 Elsevier B.V. All rights reserved.
SN 00219673
YR 2017
FD 2017-08-11
LK http://hdl.handle.net/10017/48162
UL http://hdl.handle.net/10017/48162
LA eng
DS MINDS@UW
RD 19-abr-2024