RT info:eu-repo/semantics/article
T1 In vitro antitumor and hypotensive activity of peptides from olive seeds
A1 Vásquez Villanueva, Romy Angela
A1 Muñoz Moreno, Laura
A1 Carmena Sierra, María José
A1 Marina Alegre, María Luisa
A1 García López, María Concepción
K1 Peptide
K1 Olive seeds
K1 Antitumor capacity
K1 Antihypertensive capacity
K1 Química
K1 Chemistry
AB Peptides with molecular weights below 3 kDa from the hydrolysis of olive seed proteins with Thermolysin (OS-3 kDa) have demonstrated a high in vitro hypotensive effect. This fraction has been further fractionated by semipreparative RP-HPLC to obtain 8 fractions. ACE inhibitor capacity of fractions was evaluated observing the highest capacity in fraction F5. Peptides in fraction F5 were identified by RP-HPLC- and HILIC-ESI-Q-ToF and cytotoxic effect was assessed in different cell lines. Peptide LLPSY, present in this faction, was synthesized and characterized. Despite antihypertensive capacity was not as high as in fraction F5, a significant anti-proliferative capacity on two different cancer cell lines was observed. Additional studies to assess antitumor activity confirmed that this peptide showed capability to increase the adhesion capacity of tumor cells, to decrease the migration capacity of cancer cells, and to arrest cell cycle on S phase.
SN 1756-4646
YR 2018
FD 2018-03
LK http://hdl.handle.net/10017/48153
UL http://hdl.handle.net/10017/48153
LA eng
DS MINDS@UW
RD 07-jun-2023