RT info:eu-repo/semantics/article
T1 Isolation and identification by high resolution liquid chromatography tandem mass spectrometry of novel peptides with multifunctional lipid-lowering capacity
A1 Prados Nieto, Isabel María
A1 Marina Alegre, María Luisa
A1 García López, María Concepción
K1 HPLC-MS/MS
K1 Hypolipidemia
K1 Peptides
K1 Olive by-product
K1 Química
K1 Chemistry
AB This work describes the isolation, characterization, and identification by RP-HPLC-ESI-Q-TOF of novel peptides that interfere in the fat digestion and absorption mechanisms by multiple pathways. Peptides were ultrafiltrated and peptides in the most active fraction were further separated by semipreparative RP-HPLC. Nine different subfractions were obtained observing a high amount of peptides in subfraction F3. Peptides in subfraction F3 could simultaneously reduce the solubility of cholesterol in micelles and inhibit pancreatic cholesterol esterase and pancreatic lipase, even after a simulated gastrointestinal digestion. The identification of lipid-lowering peptides has been scarcely performed and when done, low selectivity or sensitivity of employed identification techniques or conditions did not yield reliable results. Separation and detection of peptides by RP-HPLC-ESI-Q-TOF-MS was optimized and most favorable conditions were employed for the identification of peptides using de novo sequencing. Ten different peptides with 4-9 amino acids were identified. Main feature of identified peptides was the high acidity derived from a high presence of amino acids glutamic acid and aspartic acid in their sequences.
SN 0963-9969
YR 2018
FD 2018-09
LK http://hdl.handle.net/10017/48008
UL http://hdl.handle.net/10017/48008
LA eng
DS MINDS@UW
RD 26-abr-2024