RT info:eu-repo/semantics/article
T1 Enantiomeric determination of econazole and sulconazole by electrokinetic chromatography using hydroxypropyl-b-cyclodextrin combined with ionic liquids based on L-lysine and Ly-glutamic acid
A1 Marina Alegre, María Luisa
A1 Castro Puyana, María
A1 Salido Fortuna, Sandra
K1 Electrokinetic chromatography
K1 Amino acid based chiral ionic liquids
K1 Cyclodextrin
K1 Enantioseparation
K1 Econazole
K1 Sulconazole
K1 Química
K1 Chemistry
AB Two analytical methodologies based on the combined use of hydroxypropyl-beta-cyclodextrin and two different amino acid-based chiral ionic liquids (tetrabutylammonium-L-lysine or tetrabutylammonium-L-glutamic acid) in electrokinetic chromatography were developed in this work to perform the enantios-elective determination of econazole and sulconazole in pharmaceutical formulations. The influence of different experimental variables such as buffer concentration, applied voltage, nature and concentration of the ionic liquid, temperature and injection time, on the enantiomeric separation was investigated. The combination of hydroxypropyl-beta-cyclodextrin and tetrabutylammonium-L-lysine under the optimized conditions enabled to achieve the enantiomeric determination of both drugs with high enantiomeric resolution (3.5 for econazole and 2.4 for sulconazole). The analytical characteristics of the developed methodologies were evaluated in terms of linearity, precision, LOD, LOQ and recovery showing good performance for the determination of both drugs which were successfully quantitated in pharmaceutical formulations. This work reports the first analytical methodology enabling the enantiomeric determination of sulconazole in pharmaceutical formulations.
SN 00219673
YR 2020
FD 2020-06-21
LK http://hdl.handle.net/10017/47167
UL http://hdl.handle.net/10017/47167
LA eng
DS MINDS@UW
RD 02-may-2024