RT info:eu-repo/semantics/article
T1 Multi-target methodology for the screening of blood specimens in drug-facilitated sexual assault cases
A1 Prego Meleiro, Pablo
A1 Quintela Jorge, Óscar
A1 Montalvo García, Gemma
A1 García Ruiz, Carmen
K1 Drug facilitated sexual assault (DFSA)
K1 Drug facilitated crime (DFC)
K1 Blood
K1 Protein precipitation
K1 Phospholipid removal
K1 Multi-target screening
K1 Química
K1 Chemistry
AB The forensic investigation of drug-facilitated sexual assaults (DFSA) requires the toxicological examination of biological matrices to detect possible substances in victims. Whole blood is an important sample since it is theonly one available for analysis in a considerable percentage of cases. A new screening methodology was developed for the detection of 96 compounds related to DFSA in blood. Sample treatment was based on a procedurecombining protein precipitation and phospholipid removal before HPLC-MS/MS analysis. The new method operates in scheduled multiple reaction monitoring (sMRM) mode and uses the hybrid operating of the thirdquadrupole as an ion trap. The blood samples preparation was studied comparing four different protein precipitation treatments and two widely used extraction techniques as solid phase extraction and liquid-liquid extraction. The analytical performance of the screening method was evaluated in terms of selectivity and limits of detection. Finally, successful results were obtained applying the new method to the analysis of fifteen samples from real cases of the National Institute of Toxicology and Forensic Sciences.
SN 0026-265X
YR 2019
FD 2019-11
LK http://hdl.handle.net/10017/46107
UL http://hdl.handle.net/10017/46107
LA eng
DS MINDS@UW
RD 18-abr-2024