RT info:eu-repo/semantics/article T1 Time-series proteomic study of the response of HK-2 cells to hyperglycemic, hypoxic diabetic-like milieu A1 Valdés Tabernero, Alberto A1 García Pastor, Coral A1 Castro Puyana, María A1 Lucio Cazaña, Francisco Javier de A1 Marina Alegre, María Luisa K1 Química K1 Chemistry AB During diabetes, renal proximal tubular cells (PTC) are exposed to a combination of high glucose and hypoxic conditions, which plays a relevant role in the development of diabetic kidney disease (DKD). In this work, a time-series proteomic study was performed to analyse the effect of a diabetic-like microenvironment induced changes on HK-2 cells, a human cell line derived from normal proximal tubular epithelial cells. Cells simultaneouslyexposed to high glucose (25 mM) and hypoxia (1% O-2) were compared to cells in control conditionsfor up to 48 h. Diabetic conditions increased the percentage of death cells after 24 and 48 h,but no differences in the protein/cell ratio were found. The relative proteinquantification using dimethyl-labeling and UHPLC-MS/MS analysis allowed the identification of 317, 296 and 259proteins at 5, 24 and 48 h, respectively. The combination of statistical and time expression profile analyses indicated anincreased expression of proteins involved in glycolysis, and a decrease of cytoskeletal-relatedproteins. The exposure of HK-2 cells to high glucose and hypoxia reproduces some of the effects of diabetes on PTC and,with the limitations inherent toin vitrostudies, propose new mechanismsand targets to be considered in the management of DKD. SN 1932-6203 YR 2020 FD 2020-06-24 LK http://hdl.handle.net/10017/44732 UL http://hdl.handle.net/10017/44732 LA eng DS MINDS@UW RD 29-mar-2024