RT info:eu-repo/semantics/article
T1 Sustainable extraction of proteins and bioactive substances from pomegranate peel (Punica granatum L.) using pressurized liquids and deep eutectic solvents
A1 Marina Alegre, María Luisa
A1 García López, María Concepción
A1 Plaza del Moral, Merichel
A1 Hernández Corroto, Ester
K1 Pomegranate peel
K1 Pressurized liquid extraction (PLE)
K1 Deep eutectic solvents (DES)
K1 Peptide
K1 Polyphenol
K1 Mass spectrometry
K1 Química
K1 Chemistry
AB Pomegranate peel is a source of proteins, bioactive peptides, and phenolic compounds. The simultaneous extraction of these compounds required the use of polluting solvents and reagents that are non-suitable. This work targets the development of green methodologies based on pressurized liquids (PLE) or deep eutectic solvents (DES) for the extraction of these compounds. Extracts were digested with different proteolytic enzymes and different functionalities (antioxidant, hypocholesterolemia, and antihypertensive capacities) were evaluated. Highly antioxidant and hypocholesterolemic extracts and hydrolysates were obtained using PLE while high antihypertensive capacity was observed in the hydrolysates from proteins extracted using DES. Peptides and polyphenols were identified by HPLC-ESI-Q-TOF/MS. Higher amounts of peptides were shown in hydrolysates from DES extracts while hydrolysates from PLE extracts presented higher amounts of phenolic compounds. Some peptides were assigned to proteins from Punica granatum. Both green methods improved the extraction of bioactive compounds from pomegranate peel compared to the non-sustainable method. Industrial relevance: The development of green methodologies which employ sustainable solvents such as pressurized liquids (PLE) and deep eutectic solvents (DES) allows extracting proteins and bioactive compounds from pomegranate peel. In addition, these solvents improve the extraction of health beneficial compounds compared to the non-sustainable and polluting solvents. Therefore, they could be used for the development of nutraceuticals and functional foods or even in medicinal, cosmetic, and pharmaceutical preparations.
SN 1466-8564
YR 2020
FD 2020-03
LK http://hdl.handle.net/10017/44570
UL http://hdl.handle.net/10017/44570
LA eng
DS MINDS@UW
RD 24-abr-2024