RT info:eu-repo/semantics/article T1 Feasibility of cationic carbosilane dendrimers for sustainable protein sample preparation A1 Marina Alegre, María Luisa A1 García López, María Concepción A1 González García, Estefanía A1 Mata de la Mata, Francisco Javier de la A1 Sánchez-Nieves Fernández, Javier K1 Carbosilane dendrimer K1 Cationic dendrimer K1 Interaction K1 Protein enrichment K1 Protein purification K1 Química K1 Chemistry AB Protein sample preparation is the bottleneck in the analysis of proteins. The aim of this work is to evaluate the feasibility of carbosilane dendrimers functionalized with cationic groups to make easier this step. Anionic carbosilane dendrimers (sulphonate- and carboxylate-terminated) have already demonstrated their interaction with proteins and their potential in protein sample preparation. In this work, interactions between positively charged carbosilane dendrimers and different model proteins were studied when working under different pH conditions, dendrimer concentrations, and dendrimer generations. Amino- and trimethylammonium-terminated carbosilane dendrimers presented, in some cases, weak interactions with proteins. Unlike them, carbosilane dendrimers with terminal dimethylamino groups could interact, in many cases, with proteins and these interactions were affected by the pH, the dendrimer concentration, and the dendrimer generation. Moreover, dendrimer precipitation was observed at all pHs, although just second and fourth generation (2 G and 4 G) dendrimers resulted in the formation of complexes with proteins. Under experimental conditions promoting dendrimer-protein interactions, 2 G dimethylamino-terminated dendrimers were proposed as an alternative to other methods used in analytical chemistry or analysis in which an organic solvent or a resin are required to enrich/purify proteins in a complex sample. SN 0927-7765 YR 2020 FD 2020-02 LK http://hdl.handle.net/10017/44568 UL http://hdl.handle.net/10017/44568 LA eng DS MINDS@UW RD 28-mar-2024