RT info:eu-repo/semantics/article T1 Sulfonate-terminated carbosilane dendron-coated nanotubes: a greener point of view in protein sample preparation A1 González García, Estefanía A1 Gutiérrez Ulloa, Carlos Emilio A1 Mata de la Mata, Francisco Javier de la A1 Marina Alegre, María Luisa A1 García López, María Concepción K1 Single-walled carbon nanotubes K1 Sulfonate carbosilane dendrons K1 Dendron-coated nanotubes K1 Protein-dendron interaction K1 Protein extraction K1 Química K1 Chemistry AB Reduction or removal of solvents and reagents in protein sample preparation is a requirement. Dendrimers can strongly interact with proteins and have great potential as a greener alternative to conventional methods used in protein sample preparation. This work proposes the use of single-walled carbon nanotubes (SWCNTs) functionalized with carbosilane dendrons with sulfonate groups for protein sample preparation and shows the successful application of the proposed methodology to extract proteins from a complex matrix. SEM images of nanotubes and mixtures of nanotubes and proteins were taken. Moreover, intrinsic fluorescence intensity of proteins was monitored to observe the most significant interactions at increasing dendron generations under neutral and basic pHs. Different conditions for the disruption of interactions between proteins and nanotubes after protein extraction and different concentrations of the disrupting reagent and the nanotube were also tried. Compatibility of extraction and disrupting conditions with the enzymatic digestion of proteins for obtaining bioactive peptides was also studied. Finally, sulfonate-terminated carbosilane dendron-coated SWCNTs enabled the extraction of proteins from a complex sample without using non-environmentally friendly solvents that were required so far. SN 1618-2642 YR 2017 FD 2017-07-07 LK http://hdl.handle.net/10017/44488 UL http://hdl.handle.net/10017/44488 LA eng DS MINDS@UW RD 29-mar-2024