RT info:eu-repo/semantics/article
T1 Multiple protective effect of peptides released from Olea Europaea and Prunus Persica seeds against oxidative damage and cancer cell proliferation
A1 Hernández Corroto, Ester
A1 Marina Alegre, María Luisa
A1 García López, María Concepción
K1 Peptides
K1 Seed
K1 Olive
K1 Prunus
K1 Oxidative damage
K1 Cell proliferation
K1 Mass spectrometry
K1 Química
K1 Chemistry
AB The long exposition to reactive species results in oxidative stress which has been related with the development of cancer and other serious diseases. Olea europaea and Prunus persica seeds present a high protein content and preliminary results demonstrated their high potency to obtain bioactive peptides. The protective effect against oxidative damage exerted by peptides released from Olea europaea and Prunus persica seeds has been evaluated in this work. Seed hydrolysates showed protection against oxidation through four different mechanisms: inhibition of the formation of hydroxyl radicals, scavenging of free radicals, reduction of oxidizing compounds, and inhibition of lipid peroxidation. Moreover, seed hydrolysates also reduced the oxidative stress induced by an oxidizing agent on human cancer cells. Despite protection evaluated by individual mechanisms seemed to be significantly affected by the seed genotype, overall protection of seed hydrolysates was not so different. Seeds hydrolysates were not cytotoxic on normal cells but they demonstrated antiproliferative effect on human cancer cells (HeLa, PC-3, and HT-29). Peptides in all seed hydrolysates were sequenced by RP-HPLC-ESI-Q-TOF. Eighteen common peptides were observed among olive seed hydrolysates while a wider variability was observed among Prunus seed hydrolysates.
SN 0963-9969
YR 2018
FD 2018-04
LK http://hdl.handle.net/10017/44487
UL http://hdl.handle.net/10017/44487
LA eng
DS MINDS@UW
RD 18-abr-2024