RT info:eu-repo/semantics/article T1 A facile and efficient single-step approach for the fabrication of vancomycin functionalized polymer-based monolith as chiral stationary phase for nano-liquid chromatography A1 Xu, Dongsheng A1 Shao, Huikai A1 Luo, Rongying A1 Wang, Qiqin A1 Sánchez López, Elena A1 Fanali, Salvatore A1 Marina Alegre, María Luisa A1 Jiang, Z. K1 Vancomycin K1 Enantioseparation K1 Organic polymeric monolith K1 Nano-LC K1 Química K1 Chemistry AB A facile single-step preparation strategy for fabricating vancomycin functionalized organic polymer based monolith within 100 mu m fused-silica capillary was developed. The synthetic chiral functional monomer, i.e 2-isocyanatoethyl methacrylate (ICNEML) derivative of vancomycin, was co-polymerized with the cross-linker ethylene dimethacrylate (EDMA) in the presence of methanol and dimethyl sulfoxide as the selected porogens. The co-polymerization conditions were systematically optimized in order to obtain satisfactory column performance. Adequate permeability, stability and column morphology were observed for the optimized poly(ICNEML-vancomycin-co-EDMA) monolith. A series of chiral drugs were evaluated on the monolith in either several other beta-blockers. The proposed single-step approach not only resulted in a vancomycin functionalized organer polar organic-phase or reversed-phase modes. After the optimization of separation conditions, baseline or partial enantioseparation were obtained for series of drugs including thalidomide, colchicine, carteolol, salbutamol, clenbuterol andic polymer-based monolith with acceptable performance, but also significantly simplified the preparation procedure by reducing time and labor. SN 0021-9673 YR 2018 FD 2018-07-06 LK http://hdl.handle.net/10017/41506 UL http://hdl.handle.net/10017/41506 LA eng DS MINDS@UW RD 29-mar-2024