RT info:eu-repo/semantics/article
T1 A Non-Targeted Capillary Electrophoresis-Mass Spectrometry Strategy to Study Metabolic Differences in an In Vitro Model of High-Glucose Induced Changes in Human Proximal Tubular HK-2 Cells
A1 Bernardo Bermejo, Samuel
A1 Sánchez López, Elena
A1 Castro Puyana, María
A1 Benito Martínez, Selma
A1 Lucio Cazaña, Francisco Javier de
A1 Marina Alegre, María Luisa
K1 diabetic nephropathy
K1 human proximal tubular HK-2 cells
K1 capillary electrophoresis-mass spectrometry
K1 metabolomics
K1 multivariate analysis
K1 Química
K1 Chemistry
K1 Medicina
K1 Medicine
AB Diabetic nephropathy is characterized by the chronic loss of kidney function due to high glucose renal levels. HK-2 proximal tubular cells are good candidates to study this disease. The aim of this work was to study an in vitro model of high glucose-induced metabolic alterations in HK-2 cells to contribute to the pathogenesis of this diabetic complication. An untargeted metabolomics strategy based on CE-MS was developed to find metabolites affected under high glucose conditions. Intracellular and extracellular fluids from HK-2 cells treated with 25 mM glucose (high glucose group), with 5.5 mM glucose (normal glucose group), and with 5.5 mM glucose and 19.5 mM mannitol (osmotic control group) were analyzed. The main changes induced by high glucose were found in the extracellular medium where increased levels of four amino acids were detected. Three of them (alanine, proline, and glutamic acid) were exported from HK-2 cells to the extracellular medium. Other affected metabolites include Amadori products and cysteine, which are more likely cause and consequence, respectively, of the oxidative stress induced by high glucose in HK-2 cells. The developed CE-MS platform provides valuable insight into high glucose-induced metabolic alterations in proximal tubular cells and allows identifying discriminative molecules of diabetic nephropathy.
SN 1420-3049
YR 2020
FD 2020
LK http://hdl.handle.net/10017/41090
UL http://hdl.handle.net/10017/41090
LA eng
DS MINDS@UW
RD 19-abr-2024