RT info:eu-repo/semantics/article
T1 Sheathless CE-MS based metabolic profiling of kidney tissue section samples from a mouse model of Polycystic Kidney Disease
A1 Marina Alegre, María Luisa
A1 Sánchez López, Elena
A1 Lageveen-Kammeijer, G.
A1 Ramautar, R.
A1 Peters, D.
A1 Mayboroda, Oleg A.
A1 Crego Navazo, Antonio Luis
K1 Química
K1 Chemistry
AB Capillary electrophoresis-mass spectrometry (CE-MS) using a sheathless porous tip interface emerged as an attractive tool in metabolomics thanks to its numerous advantages. One of the main advantages compared to the classical co-axial sheath liquid interface is the increased sensitivity, while maintaining the inherent properties of CE, such as a high separation efficiency and low sample consumption. Specially, the ability to perform nanoliter-based injections from only a few microliters of material in the sample vial makes sheathless CE-MS a well-suited and unique approach for highly sensitive metabolic profiling of limited sample amounts. Therefore, in this work, we demonstrate the utility of sheathless CE-MS for metabolic profiling of biomass-restricted samples, namely for 20 mu m-thick tissue sections of kidney from a mouse model of polycystic kidney disease (PKD). The extraction method was designed in such a way to keep a minimum sample-volume in the injection vial, thereby still allowing multiple nanoliter injections for repeatability studies. The developed strategy enabled to differentiate between different stages of PKD and as well changes in a variety of different metabolites could be annotated over experimental groups. These metabolites include carnitine, glutamine, creatine, betaine and creatinine. Overall, this study shows the utility of sheathless CE-MS for biomass-limited metabolomics studies.
SN 2045-2322
YR 2019
FD 2019-01-28
LK http://hdl.handle.net/10017/41066
UL http://hdl.handle.net/10017/41066
LA eng
DS MINDS@UW
RD 01-may-2024