RT info:eu-repo/semantics/article
T1 An untargeted metabolomic strategy based on liquid chromatography-mass spectrometry to study high glucose-induced changes in HK-2 cells
A1 Marina Alegre, María Luisa
A1 Sánchez López, Elena
A1 Bernardo Bermejo, Samuel
A1 Castro Puyana, María
A1 Benito Martínez, Selma
A1 Lucio Cazaña, Francisco Javier de
K1 diabetic nephropathy
K1 HK-2 cells
K1 liquid chromatography-mass spectrometry
K1 metabolomics
K1 multivariate analysis
K1 Química
K1 Chemistry
AB Diabetes mellitus is a major health concern nowadays. It is estimated that 40 % of diabetics are affected by diabetic nephropathy, one of the complications derived from high glucose blood levels which can lead to chronic loss of kidney function. It is now clear that the renal proximal tubule plays a critical role in the progression of diabetic nephropathy but research focused on studying the molecular mechanisms involved is still needed. The aim of this work was to develop a liquid chromatography-mass spectrometry platform to carry out, for the first time, the untargeted metabolomic analysis of high glucose-induced changes in cultured human proximal tubular HK-2 cells. In order to find the metabolites which were affected by high glucose and to expand the metabolite coverage, intra- and extracellular fluid from HK-2 cells exposed to high glucose (25 mM), normal glucose (5.5 mM) or osmotic control (5.5 mM glucose + 19.5 mM mannitol) were analyzed by two complementary chromatographic modes: hydrophilic interaction and reversed-phase liquid chromatography. Non-supervised principal components analysis showed a good distribution among the three groups of samples. Statistically significant variables were chosen for further metabolite identification. Different metabolic pathways were affected mainly those derived from amino acidic, polyol, and nitrogenous bases metabolism.
SN 0021-967
YR 2019
FD 2019-07-05
LK http://hdl.handle.net/10017/39807
UL http://hdl.handle.net/10017/39807
LA eng
NO https://www.sciencedirect.com/science/article/pii/S002196731930247X?via%3Dihub
DS MINDS@UW
RD 18-abr-2024