RT info:eu-repo/semantics/article T1 Capsaicin Targets Lipogenesis in HepG2 Cells Through AMPK Activation, AKT Inhibition and PPARs Regulation A1 Bort Bueno, Alicia Carmen A1 Sánchez Gómez, Belén A1 Mateos Gómez, Pedro Antonio A1 Díaz-Laviada Marturet, Inés A1 Rodríguez Henche, Nieves K1 Capsaicin K1 AMPK K1 AKT K1 PPARy K1 PPARx K1 HepG2 K1 Lipid metabolism K1 Biología y Biomedicina K1 Biology AB Obesity, a major risk factor for chronic diseases such as type 2 diabetes (T2D), represents a serious primary health problem worldwide. Dietary habits are of special interest to prevent and counteract the obesity and its associated metabolic disorders, including lipid steatosis. Capsaicin, a pungent compound of chili peppers, has been found to ameliorate diet-induced obesity in rodents and humans. The purpose of this study was to examine the effect of capsaicin on hepatic lipogenesis and to delineate the underlying signaling pathways involved, using HepG2 cells as an experimental model. Cellular neutral lipids, stained with BODIPY493/503, were quantified by flow cytometry, and the protein expression and activity were determined by immunoblotting. Capsaicin reduced basal neutral lipid content in HepG2 cells, as well that induced by troglitazone or by oleic acid. This effect of capsaicin was prevented by dorsomorphin and GW9662, pharmacological inhibitors of AMPK and PPARgamma, respectively. In addition, capsaicin activated AMPK and inhibited the AKT/mTOR pathway, major regulators of hepatic lipogenesis. Furthermore, capsaicin blocked autophagy and increased PGC-1alfa protein. These results suggest that capsaicin behaves as an anti-lipogenic compound in HepG2 cells. SN 1422-0067 YR 2019 FD 2019 LK http://hdl.handle.net/10017/37531 UL http://hdl.handle.net/10017/37531 LA eng NO Fundación Tatiana Pérez De Guzmán DS MINDS@UW RD 29-mar-2024