RT info:eu-repo/semantics/article T1 Paper mill effluent decolorization by fifty Streptomyces strains. A1 Hernández Cutuli, Manuel Pascual A1 Rodríguez Bullido, Juana A1 Soliveri de Carranza, Juan A1 Copa Patiño, José Luis A1 Pérez Leblic, María Isabel A1 Arias Fernández, María Enriqueta Juana K1 article K1 bacterial growth K1 coloreffluent toxicity K1 fractionation K1 paper industry K1 ph measurement K1 reaction analysis K1 strain difference K1 streptomycesthin layer chromatography K1 Biología y Biomedicina/Biología K1 Biology AB Fifty actinomycete strains isolated from lignocellulosic substrates were examined for the ability to remove the color from a paper mill effluent obtained after semichemical alkaline pulping of wheat straw. Streptomyces sp. strains UAH 15, UAH 23, UAH 30, and UAH 51 were selected for their ability to decolorize the effluent in a liquid medium containing 1% (wt/vol) glycerol, 0.2% (wt/vol) ammonium sulfate, and 80% (vol/vol) effluent. The highest levels of decolorization achieved after the strains grew were 60 to 65%. Strains UAH 30 and UAH 51 were selected for further study because of their different patterns of effluent decolorization during growth. Fractionation of the decolorized effluent by gel permeation chromatography demonstrated that there were reductions in the levels of absorbance of the high- and medium- molecular-weight compounds. These fractions were mainly responsible for the color of the effluent, while the last fractions, the low-molecular-weight compounds, could have been responsible for the residual color of the decolorized effluent. Thin-layer chromatography revealed significant differences among the patterns of bands corresponding to the acidified supernatants obtained after precipitation of alkali-lignin from the effluent samples decolorized by different Streptomyces strains. SN 0099-2240 YR 1994 FD 1994 LK http://hdl.handle.net/10017/37006 UL http://hdl.handle.net/10017/37006 LA eng DS MINDS@UW RD 25-abr-2024