RT info:eu-repo/semantics/article T1 Microvesicles from the plasma of elderly subjects and from senescent endothelial cells promote vascular calcification A1 Alique Aguilar, Matilde A1 Ruiz Torres, María Piedad A1 Bodega Magro, Guillermo A1 Noci Luna, Victoria A1 Troyano Suárez, Nuria A1 Bohorquez Magro, María Lourdes A1 Luna Ruiz, Carlos A1 Luque, Rafael A1 Carmona, Andrés A1 Carracedo Añón, Julia María A1 Ramírez Chamond, Manuel Rafael K1 Aging K1 Senescence K1 Microvesicles K1 Vascular calcification K1 Endothelial cells K1 Vascular smooth muscle cells K1 Biología y Biomedicina K1 Biology AB Vascular calcification is commonly seen in elderly people, though it can also appear in middle-aged subjects affected by premature vascular aging. The aim of this work is to test the involvement of microvesicles (MVs) produced by senescent endothelial cells (EC) and from plasma of elderly people in vascular calcification. The present work shows that MVs produced by senescent cultured ECs, plus those found in the plasma of elderly subjects, promote calcification in vascular smooth muscle cells. Only MVs from senescent ECs, and from elderly subjects' plasma, induced calcification. This ability correlated with these types of MVs' carriage of: a) increased quantities of annexins (which might act as nucleation sites for calcification), b) increased quantities of bonemorphogenic protein, and c) larger Ca contents. The MVs of senescent, cultured ECs, and those present in the plasma of elderly subjects, promote vascular calcification. The present results provide mechanistic insights into the observed increase in vascular calcification-related diseases in the elderly, and in younger patients with premature vascular aging, paving the way towards novel therapeutic strategies. SN 1945-4589 YR 2017 FD 2017 LK http://hdl.handle.net/10017/35846 UL http://hdl.handle.net/10017/35846 LA eng NO Instituto de Salud Carlos III DS MINDS@UW RD 29-mar-2024