RT info:eu-repo/semantics/article
T1 Mesoporous Silica Nanoparticles Decorated with Carbosilane Dendrons as New Non-viral Oligonucleotide Delivery Carriers
A1 Gómez Ramírez, Rafael
A1 Martínez Martínez, Ángel
A1 Fuentes Paniagua, María Elena
A1 Baeza García, Alejandro
A1 Mata de la Mata, Francisco Javier de la
A1 Sánchez-Nieves Fernández, Javier
A1 Ciuendez, Monica
A1 González Mancebo, Blanca
A1 Vallet  Reig, Maria
K1 Carbosilane dendrons
K1 Mesoporous silica nanoparticles
K1 Gene transfection
K1 Nonviral vectors
K1 Química
K1 Chemistry
AB A novel nanosystem based on mesoporous silica nanoparticles covered with carbosilane dendrons grafted on their external surface is reported. This system is able to transport single oligonucleotide strands into cells, avoiding the electrostatic repulsion between the cell membrane and the negatively charged nucleic acids thanks to the cationic charge provided by the dendron coating in physiological conditions. Moreover, the presence of the highly ordered pore network inside the silica matrix would make possible to allocate other therapeutic agents within the mesopores with the aim of achieving a double delivery. First, carbosilane dendrons of second and third generation pos- sessing ammonium or tertiary amine groups as peripheral functional groups were prepared. Hence, different strategies were tested in order to obtain their suitable grafting on the nanoparticles outer surface. As nucleic acid model, a single stranded DNA oligonucleotide tagged with a fluorescent Cy3 moiety was used to evaluate the DNA adsorption capacity. The hybrid material functionalized with the third generation of neutral dendron showed excellent DNA binding properties. Finally, the cytotoxicity as well as the capability to deliver DNA into cells, was tested using a human osteoblast-like cell line, achieving good levels of internalization of the vector DNA/carbosilane dendron functionalized material without affecting cellular viability.
PB Wiley
SN 0947-6539
YR 2015
FD 2015-09-10
LK http://hdl.handle.net/10017/33947
UL http://hdl.handle.net/10017/33947
LA eng
NO Ministerio de Economía y Empresa
DS MINDS@UW
RD 27-abr-2024