RT info:eu-repo/semantics/article
T1 Glucocorticoid receptor changes its cellular location with breast cancer development.
A1 Paniagua Gómez-Álvarez, Ricardo
A1 Fraile Laiz, Benito
A1 Arenas Jiménez, María Isabel
A1 Lucio Cazaña, Francisco Javier de
A1 Conde Martín, María Isabel
K1 GR
K1 MR
K1 COX-2
K1 Breast cancer
K1 Biología
K1 Biology
K1 Genética
K1 Genetics
K1 Ciencia
K1 Science
K1 Adult
K1 Aged
K1 Aged, 80 and over
K1 Breast
K1 Breast Neoplasms
K1 Carcinoma in Situ
K1 Carcinoma, Ductal
K1 Carcinoma, Lobular
K1 Cyclooxygenase 2
K1 Female
K1 Fibroadenoma
K1 Humans
K1 Hyperplasia
K1 Metaplasia
K1 Middle Aged
K1 Receptors, Glucocorticoid
K1 Receptors, Mineralocorticoid
AB Glucocorticoids play a major role in attenuation of the inflammatory response and they are useful in the primary combination chemotherapy of breast cancer, since in vitro studies have demonstrated an antiproliferative effect in human breast cancer cells. In contrast, it was recently shown that glucocorticoids protect against apoptotic signals evoked by cytokines, cAMP, tumour suppressors, and death genes in mammary gland epithelia. Their actions are mediated by intracellular receptor (GR) that functions as a hormone-dependent transcription factor; however, no previous studies have been focused on GR expression in different pathologies of the human breast, and the possible relationship with that of mineralocorticoid receptor (MR) and COX-2. Also, the role of these proteins on tumoral breast epithelial cells remains unclear. Therefore, we examined GR, MR and COX-2 expression by immunohistochemistry and Western blot techniques in 142 samples of human breast obtained by total or partial mastectomy. We found that the percentage of positive patients presenting nuclear immunoreaction to GR decreased with tumor development, while all samples analyzed showed cytoplasmic immunoreactions to MR. All positive samples to COX-2 antibody showed cytoplasmic location, a higher immunoreaction being observed in benign breast diseases than in carcinomatous lesions. Thus, breast cancer progression is associated with the accumulation of GR in the cytoplasm of tumoral cells and the decrease of COX-2 expression.
PB Sercrisma International S.L.
SN 0213-3911
YR 2008
FD 2008-07-13
LK http://hdl.handle.net/10017/32724
UL http://hdl.handle.net/10017/32724
LA eng
DS MINDS@UW
RD 26-abr-2024