RT info:eu-repo/semantics/article
T1 Pancreatic changes in somatostatin content and receptor-effector system after intrapancreatic injection of 5,7-dihydroxytryptamine
A1 Muñoz Acedo, Gema
A1 Álvaro Alonso, Itziar
A1 Arilla Ferreiro, Eduardo
K1 Acinar cells
K1 Guinea pig
K1 Adenylate cyclase
K1 Amylase secretion
K1 Exocrine pancreas
K1 5-HT1P receptors
K1 Rat brain
K1 Binding
K1 Identification
K1 Forskolin
K1 Bioquímica
K1 Biochemistry
K1 Science
K1 Ciencia
AB To date, it is unknown whether intrapancreatic serotonergic nerves can influence pancreatic somatostatin (SS) content and the SS receptor/effector system in the exocrine pancreas. In this study, the intrapancreatic serotonergic nerves were chemically ablated by injecting a specific serotonin (5-HT) neurotoxin, 5,7-dihydroxytryptamine (5,7-DHT), into the substance of the gland. Three days after the injection, the 5-HT-like immunoreactive levels in the pancreas were reduced by more than 85% whereas somatostatin-like immunoreactive levels had increased (86%). The number of SS receptors in the pancreatic acinar cell membranes of the 5,7-DHT-treated rats was also increased (72%). No significant differences were seen in basal or forskolin-stimulated adenylate cyclase (AC) enzyme activities in the control and the 5,7-DHT-treated groups. In spite of the increase in the number of SS receptors in the pancreatic acinar cell membranes of 5,7-DHT-treated rats, SS caused a significantly lower inhibition of AC activity in these membranes. This finding is related to the observed decrease of a 41 kD pertussis toxin-sensitive substrate, presumably the αi subunit of the guanine nucleotide inhibitory protein, in pancreatic acinar cell membranes 3 days after intrapancreatic 5,7-DHT administration when compared with the corresponding controls. The functions of pancreatic serotonergic nerves seem to be associated with enteropancreatic communication. These data together with the present results suggest that pancreatic SS content and the SS receptor/effector system in the exocrine pancreas may be regulated by enteropancreatic serotonergic nerve fibers and may participate in enteropancreatic reflexes.
SN 0022-0795
YR 1995
FD 1995
LK http://hdl.handle.net/10017/3216
UL http://hdl.handle.net/10017/3216
LA eng
NO Dirección General de Investigación Científica y Técnica
DS MINDS@UW
RD 25-abr-2024