RT info:eu-repo/semantics/article
T1 Effect of cysteamine on cytosolic somatostatin binding-sites in rabbit duodenal mucosa
A1 González Guijarro, Luis A.
A1 López Ruiz, María del Pilar
A1 Bodega Magro, Guillermo
A1 Prieto Villapún, Juan Carlos
A1 Arilla Ferreiro, Eduardo
K1 Somatostatin
K1 Rabbit
K1 Mucosa
K1 Bioquímica
K1 Biochemistry
K1 Science
K1 Ciencia
AB Administration of cysteamine in rabbits elicited a rapid depletion of both duodenal mucosa and plasma somatostatin. A significant reduction was observed within 5 min, returning toward control values by 150 min. The depletion of somatostatin was associated with an increase in the binding capacity and a decrease in the affinity of both high- and low-affinity binding sites present in cytosol of duodenal mucosa. Incubation of cytosolic fraction from control rabbits with 1 mM cysteamine did not modify somatostatin binding. Furthermore, addition of cysteamine at the time of binding assay did not affect the integrity of 125I-Tyr11-somatostatin. It is concluded that in vivo administration of cysteamine to rabbits depletes both duodenal mucosa and plasma somatostatin and leads to up-regulation of duodenal somatostatin binding sites.
PB Academic Press
SN 0014-4800
YR 1987
FD 1987
LK http://hdl.handle.net/10017/2472
UL http://hdl.handle.net/10017/2472
LA eng
NO Instituto de Salud Carlos III
DS MINDS@UW
RD 23-abr-2024