RT info:eu-repo/semantics/article
T1 Identification by hydrophilic interaction and reversed-phase liquid chromatography-tandem mass spectrometry of peptides with antioxidant capacity in food residues
A1 Marina Alegre, María Luisa
A1 García López, María Concepción
A1 Vásquez Villanueva, Romy
K1 Hydrophilic interaction
K1 Reversed-phase
K1 Liquid chromatography–tandem mass spectrometry
K1 Peptides
K1 Antioxidant
K1 Peach seed
K1 CIENCIA
K1 Química
K1 SCIENCE
K1 Chemistry
AB HILIC- and RP-HPLC-ESI-Q-TOF identification of bioactive peptides with antioxidant capacity in peach by-products was carried out. Peach seeds contain more than 40% of proteins (as dried and defatted basis) and could constitute a cheap source of bioactive peptides. Extracted proteins were digested using four different commercial enzymes. Five assays based on different antioxidant mechanisms were employed for a reliable evaluation of the antioxidant capacity of the extracts. Thermolysin enzyme originated the extract with the most favorable antioxidant capacity. Probably due to a synergic effect among antioxidant peptides, it was not possible to find a peptide fraction with a higher antioxidant capacity than the whole extract. Eighteen peptides were identified in the whole hydrolysate when combining HILIC- and RP-HPLC-ESI-Q-TOF. A high percentage of hydrophobic amino acids were observed within their sequences which is a characteristic feature of the antioxidant nature of peptides.
PB Elsevier
SN 0021-9673
YR 2016
FD 2016
LK http://hdl.handle.net/10017/24608
UL http://hdl.handle.net/10017/24608
LA eng
DS MINDS@UW
RD 06-feb-2023