RT info:eu-repo/semantics/article
T1 Identification by hydrophilic interaction and reversed-phase liquid chromatography-tandem mass spectrometry of peptides with antioxidant capacity in food residues
A1 Marina Alegre, María Luisa
A1 García López, María Concepción
A1 Vásquez Villanueva, Romy Ángela
K1 Hydrophilic interaction
K1 Reversed-phase
K1 Liquid chromatography–tandem mass spectrometry
K1 Peptides
K1 Antioxidant
K1 Peach seed
K1 Ciencia
K1 Química
K1 Science
K1 Chemistry
AB HILIC- and RP-HPLC-ESI-Q-TOF identification of bioactive peptides with antioxidant capacity in peach by-products was carried out. Peach seeds contain more than 40% of proteins (as dried and defatted basis) and could constitute a cheap source of bioactive peptides. Extracted proteins were digested using four different commercial enzymes. Five assays based on different antioxidant mechanisms were employed for a reliable evaluation of the antioxidant capacity of the extracts. Thermolysin enzyme originated the extract with the most favorable antioxidant capacity. Probably due to a synergic effect among antioxidant peptides, it was not possible to find a peptide fraction with a higher antioxidant capacity than the whole extract. Eighteen peptides were identified in the whole hydrolysate when combining HILIC- and RP-HPLC-ESI-Q-TOF. A high percentage of hydrophobic amino acids were observed within their sequences which is a characteristic feature of the antioxidant nature of peptides.
PB Elsevier
SN 0021-9673
YR 2016
FD 2016
LK http://hdl.handle.net/10017/24608
UL http://hdl.handle.net/10017/24608
LA eng
DS MINDS@UW
RD 26-abr-2024