RT info:eu-repo/semantics/article
T1 Fractionation and identification of antioxidant and angiotensin-converting enzyme-inhibitory peptides obtained from plum (Prunus domestica L.) stones
A1 Marina Alegre, María Luisa
A1 González García, Estefanía
A1 Puchalska, Patrycja Anna
A1 García López, María Concepción
K1 Plum stone
K1 ACE-inhibitory peptides
K1 Antioxidant peptides
K1 Hydrophilic interaction chromatography
K1 Mass spectrometry
K1 Ciencia
K1 Química
K1 Science
K1 Chemistry
AB Peptides with antioxidant and ACE-inhibitory capacities have been obtained from a plum processing by-product. The aim of this work was to fractionate most active plum hydrolysates, to identify peptides in these fractions, and to evaluate their resistance to gastrointestinal enzymes. Highest antioxidant capacity was observed in the whole Alcalase hydrolysate while peptides yielding the highest ability to inhibit ACE were concentrated in the fraction <3 kDa from the Thermolysin hydrolysate. RP-HPLC–ESI-MS/MS enabled the identification of 13 and 7 potential antioxidant and ACE inhibitory peptides, respectively, in these fractions. Nevertheless, RP-HPLC alone did not guarantee the successful separation of all peptides. The combination of RP-HPLC and hydrophilic interaction liquid chromatography (HILIC) coupled to ESI-Q-TOF-MS/MS enabled the identification of all peptides. Five potential antioxidant and three potential antihypertensive peptides could be additionally identified by HILIC. A total of 7 potential antioxidant peptides and 3 potential antihypertensive peptides resisted a simulated gastrointestinal digestion.
PB Elsevier
SN 1756-4646
YR 2015
FD 2015
LK http://hdl.handle.net/10017/24159
UL http://hdl.handle.net/10017/24159
LA eng
NO Novozymes Spain S.A.
DS MINDS@UW
RD 20-abr-2024