RT info:eu-repo/semantics/article
T1 Somatostatin binding capacity, guanylate cyclase and tyrosine phosphatase activities during pancreatic proliferation in the rat induced by gastrectomy
A1 Rodríguez Martín, Eulalia
A1 Valencia Torralba, Ana M.
A1 Colás Escudero, María Begoña
A1 García Escribano, Carmen
A1 Rodríguez Puyol, Manuel
A1 Susini, Christiane
A1 Arilla Ferreiro, Eduardo
K1 Gastrectomy
K1 Guanylate cyclase
K1 Somatostatin receptors
K1 Pancreatic acinar membranes
K1 Somatostatin like-immunoreactivity
K1 Rat
K1 Bioquímica
K1 Biochemistry
K1 Science
K1 Ciencia
AB Gastrectomy increased pancreatic growth and this effect was associated with an increase in the number of somatostatin-14 (SS) receptors (146% of control) without altering their affinity. SS increased guanylate cyclase activity twofold in pancreatic acinar membranes from gastrectomized rats. The gastrectomy decreased pancreatic SS-like immunoreactivity (SS-LI) content (55% of control levels) and tyrosine phosphatase activity (74% of control levels). Administration of proglumide (20 mg/kg, IF), a gastrin/cholecystokinin (CCK) receptor antagonist, suppressed the inhibitory effect of gastrectomy on basal tyrosine phosphatase activity and SS-LI content, which returned to control levels. Furthermore, proglumide suppressed the increase of the number of SS receptors and of SS-stimulated guanylate cyclase activity induced by gastrectomy. All this suggests that pancreatic acinar cell growth is associated with upregulation of SS receptors, which could represent a mechanism promoted by the cell to negatively regulate the mitogenic activity of pancreatic growth factors such as CCK. In addition, the results also suggest that the negative regulation of tyrosine phosphatase activity may be important in the events involved in the pancreatic hyperplasia observed after gastrectomy.
PB Elsevier
SN 0196-9781
YR 1995
FD 1995
LK http://hdl.handle.net/10017/2318
UL http://hdl.handle.net/10017/2318
LA eng
NO Ministerio de Educación y Ciencia
DS MINDS@UW
RD 25-abr-2024