RT info:eu-repo/semantics/article T1 Adenylate cyclase activity during exocrine pancreatic proliferation in the rat A1 Álvaro Alonso, Itziar A1 Boyano Adánez, María del Carmen A1 Martín Espinosa, Ángela A1 Arilla Ferreiro, Eduardo K1 Adenylate cyclase K1 Small bowel resection K1 Pentagastrin K1 Pancreas K1 Hyperplasia K1 Bioquímica K1 Biochemistry K1 Science K1 Ciencia AB Adenylate cyclase activity in pancreatic acinar cell membranes was determined in rats that had undergone a treatment with pentagastrin (250 μg/kg, intraperitoneal three times daily) for 1 week or that had undergone small bower resection (90%) and were sacrified at 2 weeks, 1 month and 6 months after intervention. Both treatments are potent stimulators of pancreatic acinar cell proliferation. Adenylate cyclase activity was similar under basal conditions and after the diterpene forskolin stimulation in pancreatic acinar membranes from all groups studied. The ability of low concentrations of the stable GTP analogue, 5'-guanylylimidodiphosphate (Gpp[NH]p) to inhibit forskolin- stimulated adenylate cyclase activity was decreased in pancreatic acinar membranes from enterectomized rats at 2 weeks and 1 month after the operation and returned to control values at 6 months after enterectomy. Stimulation of adenylate cyclase by high concentration of Gpp[NH]p or by secretin (10-8 M) was higher in both pancreatic hyperplasia conditions as compared with control animals. These findings suggest that the coupling efficiency of the G(s) protein to adenylate cyclase from pancreatic acinar membranes is enhanced without any alterations in the catalytic activity of the enzyme during pancreatic proliferation. In addition, it is possible that the highly regulated pancreatic acinar adenylate cyclase activity may be necessary to regulate pancreatic acinar cell proliferation. PB Elsevier SN 0024-3205 YR 1995 FD 1995 LK http://hdl.handle.net/10017/2251 UL http://hdl.handle.net/10017/2251 LA eng NO Ministerio de Educación y Ciencia DS MINDS@UW RD 29-mar-2024