RT info:eu-repo/semantics/article T1 Effect of nitric oxide on the somatostatinergic system in the rat exocrine pancreas A1 Rodríguez Martín, Eulalia A1 Muñoz Acedo, Gema A1 Puebla Jiménez, Lilian A1 Arilla Ferreiro, Eduardo K1 Nitric oxide K1 Somatostatin receptor K1 Adenylyl cyclase K1 Somatostatin-like immunoreactivity K1 Pancreatic acinar membrane K1 Rat K1 Bioquímica K1 Biochemistry K1 Science K1 Ciencia AB Nitric oxide (NO) and somatostatin (SS) are two important mediators of the exocrine and endocrine pancreas, exerting opposite effects on this organ. There is strong evidence suggesting an interaction between pancreatic NO and SS. The aim of this study was to determine whether L-arginine (L-Arg), the substrate for NO synthase (NOS), and Nω-nitro-L-arginine methyl ester (L-NAME), a NOS inhibitor, regulate pancreatic somatostatin-like immunoreactivity (SSLI) content and the SS mechanism of action in pancreatic acinar cell membranes. L-Arg (150 mg/kg, intraperitoneally (i.p.)), L-NAME (50 mg/kg, i.p.) or L-NAME plus L-Arg were injected twice daily at 8 h intervals for 8 days. L-Arg decreased pancreatic SSLI content as well as the number of SS receptors in pancreatic acinar cell membranes whereas L-NAME increased both parameters. The stable SS analogue SMS 201-995 induced a significantly lower inhibition of forskolin-stimulated adenylyl cyclase activity in pancreatic acinar cell membranes from L-Arg-treated rats whereas an increased inhibition was observed in pancreatic acinar membranes from L-NAME-treated rats. These results indicate that the NO system may contribute to the regulation of the pancreatic somatostatinergic system. PB Elsevier SN 0006-3002 YR 1999 FD 1999 LK http://hdl.handle.net/10017/2175 UL http://hdl.handle.net/10017/2175 LA eng NO Ministerio de Educación y Cultura DS MINDS@UW RD 28-mar-2024