RT info:eu-repo/semantics/article T1 Determination of betaines in vegetable oils by capillary electrophoresis tandem mass spectrometry. Application to the detection of olive oil adulteration with seed oils. A1 Sánchez Hernández, Laura A1 Castro Puyana, María A1 Marina Alegre, María Luisa A1 Crego Navazo, Antonio Luis K1 Betaines K1 Capillary electrophoresis K1 Corn oil K1 Olive oil K1 Soybean oil K1 Sunflower oil K1 Tandem mass spectrometry K1 Ciencia K1 Química K1 Science K1 Chemistry AB A capillary electrophoresis-tandem mass spectrometry methodology enabling thesimultaneous determination of betaines (glycine betaine, trigonelline, proline betaine andtotal content of carnitines) in vegetable oils was developed. Betaines were derivatizedwith butanol previous to their baseline separation in 10 min using a 0.1 M formic acidbuffer at pH 2.0. Ion trap conditions were optimized in order to maximize selectivity andsensitivity. Analytical characteristics of the proposed method were established byevaluating its selectivity, linearity, precision (RSDs ranged from 4.8% to 10.7% forcorrected peak areas), accuracy by means of recovery studies (from 80% to 99%) andLODs and LOQs at 0.1 ppb level. The method was applied to the determination of theselected betaines in seed oils and extra virgin olive oils. MS2 experiments provided thefingerprint fragmentation for all betaines studied in seed oils. In extra virgin olive oils,carnitines were not detected being possible to propose them as a feasible novel marker forthe detection of adulterations of olive oils. Application of the developed method to theanalysis of different mixtures of extra virgin olive oil with seed oil (between 2-10 %)enabled the detection and quantitation of the total content of carnitines. The resultsobtained show the high potential of the developed method for the authentication andquality control of olive oils. PB Wiley-VCH SN 0173-0835 YR 2011 FD 2011 LK http://hdl.handle.net/10017/21130 UL http://hdl.handle.net/10017/21130 LA eng NO The final publication is available at Springer via http://dx.doi.org/10.1002/elps.201100005 NO Authors thank the Spanish Ministry of Science and Innovation (project CTQ2009- 09022) and the Comunidad Autónoma of Madrid (Spain) and european funding from FEDER programme (project S2009/AGR-1464, ANALISYC-II). They also thank the University of Alcalá and the Comunidad Autónoma of Madrid for project CCG10- UAH/AGR-5950. Laura Sánchez-Hernández thanks the Comunidad Autónoma of Madrid for her research contract. DS MINDS@UW RD 29-mar-2024