RT info:eu-repo/semantics/article T1 Cell-based high-throughput screening assay for identification of G-protein-coupled receptors agonists and antagonists A1 Bejarano Prudencio, Alberto K1 Ca2+ mobilization K1 GPCRs K1 FLIPR K1 Fluorescent Ca2+ dye K1 High-throughput screening K1 Biología y Biomedicina K1 Biology K1 Farmacia K1 Pharmacy AB The GPCRs are the target of a significant portion of all approved drugs and also represent an important parcel of potential drug targets for new indications. Activation of GPCRs can be detected by the utilization of functional cell-based assays like measuring transient intracellular calcium mobilization. The emergence of the fluorescent imaging plate reader and Ca2+-sensitive fluorescent dyes has made the high-content screening of GPCR possible. Here was applied a dual automated FLIPR platform to testing a large collection of compounds to identification of both agonist and antagonists form a single screen of GPCR PB InnovARTE, Grupo Docente. Universidad de Alcalá SN 1886-8746 YR 2013 FD 2013 LK http://hdl.handle.net/10017/15202 UL http://hdl.handle.net/10017/15202 LA eng DS MINDS@UW RD 29-mar-2024