RT info:eu-repo/semantics/article
T1 Fast enantiomeric separation of uniconazole and diniconazole by electrokinetic chromatography using an anionic cyclodextrin: application to the determination of analyte-selector apparent binding constants for enantiomers
A1 Martín Biosca, Yolanda
A1 García Ruiz, Carmen
A1 Marina Alegre, María Luisa
K1 Uniconazole
K1 Diniconazole
K1 Electrokinetic chromatography
K1 Ciencia
K1 Química analítica e industrial
K1 Science
K1 Chemistry, analytic and technical
AB The enantiomeric resolution of the fungicides uniconazole and diniconazole was performed using electrokinetic chromatography with cyclodextrins as pseudostationary phase (CD-EKC). A systematic evaluation of several chiral selectors was made. The anionic derivative carboxymethylated-gamma-cyclodextrin (CM-gamma-CD) was found to be the most appropriate for the enantioseparation of fungicides among all cyclodextrins tested. The influence of some experimental conditions such as nature and buffer pH, chiral selector concentration, and temperature on the enantiomeric separation of the compounds studied was also investigated. The use of a 50 mM phosphate buffer (pH 6.5) containing 5 mM CM-gamma-CD and a temperature of 50 degrees C enabled the baseline enantioresolution of mixtures of uniconazole and diniconazole in less than 5 min. In addition, apparent binding constants for each enantiomer-CM-gamma-CD pair at several temperatures, as well as thermodynamic parameters for binding were calculated.
PB John Wiley & Sons
SN 0173-0835
YR 2000
FD 2000
LK http://hdl.handle.net/10017/1366
UL http://hdl.handle.net/10017/1366
LA eng
NO Comunidad de Madrid
DS MINDS@UW
RD 20-abr-2024