RT info:eu-repo/semantics/article T1 Laser-induced fluorescence detection at 266 nm in capillary electrophoresis Polycyclic aromatic hydrocarbon metabolites in biota A1 Kuijt, J. A1 García Ruiz, Carmen A1 Stroomberg, G.J. A1 Marina Alegre, María Luisa A1 Ariese, Freek A1 Brinkman, U.A.Th. A1 Gooijera, C. K1 Laser-induced fluorescence detection K1 Detection, electrophoresis K1 Polynuclear aromatic hydrocarbons K1 Ciencia K1 Química analítica e industrial K1 Science K1 Chemistry, analytic and technical AB The separation of five phenolic polycyclic aromatic hydrocarbon metabolites (hydroxy-PAHs) has been performed by cyclodextrin-modified micellar electrokinetic chromatography (CD-MEKC) using a 30 mM borate buffer (pH 9.0) containing 60 mM sodium dodecyl sulfate and varying concentrations of γ-cyclodextrin (γ-CD). A concentration of 12.5 mM γ-CD was found to provide a baseline separation of the five hydroxy-PAHs. We applied conventional fluorescence and laser-induced fluorescence (LIF) detection, using a new, small-size, quadrupled Nd–YAG laser emitting at 266 nm. The best limits of detection, in the low ng/ml range, were achieved using LIF detection. For all analytes, linearity was observed up to ca. 100 ng/ml. As an application, conjugated pyrene metabolites in hepatopancreas samples from the terrestrial isopods Oniscus asellus and Porcellio scaber were separated and detected. Finally, flatfish bile samples from individuals exposed to polluted sediment or crude oil, which were part of an interlaboratory study, were analyzed by CD-MEKC with conventional fluorescence and LIF detection to determine the 1-hydroxypyrene concentrations. PB Elsevier SN 0021-9673 YR 2001 FD 2001 LK http://hdl.handle.net/10017/1352 UL http://hdl.handle.net/10017/1352 LA eng NO The authors wish to thank the Dutch Foundation for the Advancement of Science (NOW) for financial support and equipment (grant No. 344-006). Also, the technical assistance of Mr. J. Buijs is much appreciated. DS MINDS@UW RD 29-mar-2024