RT info:eu-repo/semantics/article
T1 Enantioselective separation of the sunscreen agent 3-(4-methylbenzylidene)-camphor by electrokinetic chromatography: Quantitative analysis in cosmetic formulations
A1 Gómara, Belén
A1 García Ruiz, Carmen
A1 Marina Alegre, María Luisa
K1 Cosmetic creams
K1 Cyclodextrins
K1 Electrokinetic chromatography
K1 Enantioselective analysis
K1 3-(4-Methylbenzylidene)-camphor
K1 Ciencia
K1 Química analítica e industrial
K1 Science
K1 Chemistry, analytic and technical
AB 3-(4-Methylbenzylidene)-camphor (MBC) is a chiral sunscreen agent used in cosmetic products. In this work, the enantioseparation of MBC has been performed by EKC and applied to the analysis of the MBC enantiomers in cosmetic creams. Different experimental conditions (type and concentration of the chiral selector, temperature, and sample solvent) have been optimized. Due to the neutral nature of this compound, anionic CD derivatives were investigated as chiral selectors. Carboxymethylated-β-CD (CM-β-CD) showed the highest chiral separation power, observing that a 15 mM concentration of this CD at a working temperature of 15°C enabled to obtain the highest enantioresolution. However, under these conditions, tailing of peaks obtained for the enantiomers was observed. The addition of increasing concentrations of the neutral α-CD to CM-β-CD at a 15 mM concentration in a 100 mM borate buffer at pH 9.0 improved the enantiomeric separation and decreased peak tailing. The use of DMF for the total dissolution of the cosmetic creams, and methanol:water (1:1 v/v) for appropriate dilution enabled to observe good shape and size for the peaks of the MBC enantiomers. After optimizing a method for the preconditioning of the capillary, the analytical characteristics of the chiral separation method for the analysis of MBC were investigated. Linearity, LODs and LOQs, precision (instrumental repeatability, method repeatability, intermediate precision), accuracy, and selectivity were evaluated. The method was applied to analyze MBC enantiomers contained in two commercial cosmetic creams containing racemic MBC and to study the skin absorption of this compound with time.
PB John Wiley & Sons
SN 0173-0835
YR 2005
FD 2005
LK http://hdl.handle.net/10017/1348
UL http://hdl.handle.net/10017/1348
LA eng
NO Authors thank the Ministry of Science and Technology (Spain) for the research project BQU2003-03638. C.G.-R. thanks the Ministry of Science and Technology for the Ramón y Cajal program (RYC-2003-001). B.G. thanks CSIC and the RYC-2003-001 project for the grants.Authors also thank Cyclolab (Budapest, Hungary) for the kind gift of CE-b-CD used in this work
DS MINDS@UW
RD 30-abr-2024