RT info:eu-repo/semantics/article
T1 Monitoring the stereoselectivity of biodegradation of chiral polychlorinated biphenyls using electrokinetic chromatography
A1 García Ruiz, Carmen
A1 Andrés Herranz, Román
A1 Valera, José Luis
A1 Laborda Rodríguez, Fernando
A1 Marina Alegre, María Luisa
K1 Chiral polychlorinated biphenyls
K1 Stereoselectivity
K1 Biodegradation
K1 Electrokinetic chromatograph
K1 Ciencia
K1 Química analítica e industrial
K1 Science
K1 Chemistry, analytic and technical
AB A study of the biodegradation of several chiral polychlorinated biphenyls (PCBs,IUPAC numbers 45, 88, 91, 95, 136, 144, 149, and 176) by a naturally occurring soilbacterium as well as the stereoselectivity of such process has been performed usingElectrokinetic Chromatography with cyclodextrins (CD-EKC). In order to achieve theanalysis of chiral PCBs, a CD-EKC system based on 50 mM 2-morpholinoethanesulfonicacid (MES) buffer (pH 6.5) containing 2 M urea, 20 mM carboxymethylated ccyclodextrin(CM-c-CD), and 10 mM b-cyclodextrin (b-CD) was used. First, the percentagedegradation of PCBs by the microorganism used in this work was measuredfor different incubation times (22, 46, 71, 93, 165, 213, and 262 hours). The resultsshowed a high degree of biodegradation (from 61% to 94%) for the eight PCBs studiedafter 262 hours of incubation with the microorganism, degradation being favouredfor the least chlorinated congeners. Secondly, measurement of the atropisomericratio of the PCB atropisomers during the biodegradation process revealed no significantvariation of the atropisomeric ratios with the degradation time, indicating a nonstereoselectivedegradation of PCBs by the microorganism
PB John Wiley & Sons
SN 1615-9306
YR 2002
FD 2002
LK http://hdl.handle.net/10017/1328
UL http://hdl.handle.net/10017/1328
LA eng
NO The authors thank the Comunidad Autónoma de Madrid (Spain)  for projects 07 M/0640/1997 and 07 M/0049/1998.
DS MINDS@UW
RD 28-abr-2024