RT info:eu-repo/semantics/article
T1 Reversed-phase high-performance liquid chromatography applied to the determination of soybean proteins in commercial heat-processed meat products
A1 García López, María Concepción
A1 Domínguez, Mar
A1 García Ruiz, Carmen
A1 Marina Alegre, María Luisa
K1 Soybean proteins
K1 Heat-processed meat products
K1 High-performance liquid chromatography
K1 Ciencia
K1 Química analítica e industrial
K1 Science
K1 Chemistry, analytic and technical
AB A reversed-phase chromatographic method has been developed and optimised in order to detect and quantitate soybean proteins in commercial heat-processed meat products. The optimised conditions consisted of a linear binary gradient tetrahydrofurane–water–0.05% trifluoroacetic acid at a flow rate of 1 mL/min. Meat products were defatted with acetone and soybean proteins were extracted with a buffered solution at pH 9.60. The injection of this extract into the chromatographic system enabled the detection of soybean proteins in heat-processed meat products in about 12 min. The method enabled the detection and quantitation of additions of 0.38% (w/w) and 0.63% (w/w), respectively, of soybean proteins (related to 10 g of initial product). The method has been proven to be precise with relative standard deviations (R.S.D.) for repeatability, intermediate precision, and internal reproducibility lower to 7.0%. Recoveries obtained for spiked meat products were close to 100% and no matrix interferences were observed. The application of the method to commercial heat-processed meat products in whose formulation soybean proteins were present yielded soybean protein contents ranging from 0.90% to 1.54%, below the maximum levels established by regulations.
PB Elsevier
SN 0003-2670
YR 2006
FD 2006
LK http://hdl.handle.net/10017/1320
UL http://hdl.handle.net/10017/1320
LA eng
NO The authors thank the Comunidad Autonoma de Madrid (Spain) for project 07G/0025/2003. Dr. C. García-Ruiz alsothanks the Ministry of Science and Technology (Spain) for her contract from the Ramón y Cajal program (RYC-2003-001).
DS MINDS@UW
RD 24-abr-2024