Phosphomonoesterases and cholinesterases from Taenia pisiformis cysticerci
Authors
Giménez Pardo, Consuelo; Martinez Grueiro, M.M.; Gomez-Barrio, A.; Martinez Fernandez, A.R.; Rodríguez Caabeiro, Filomena CrisantaDate
2004-09Bibliographic citation
Helminthologia, 2004, v. 41, n. 3, p. 115-120
Keywords
acid phosphatase
alkaline phosphatase
acetylcholinesterase
metacestode
Taenia pisiformis
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
© The Authors
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Access rights
info:eu-repo/semantics/openAccess
Abstract
Phosphohydrolase and cholinesterase activities were found in the cytosolic fraction of Taenia pisiformis cysticerci from naturally infected hares. By using p-nitrophenyl phosphate as substrate, mainly acid (pH 5:0) but also alkaline (pH 10.0) phosphatase activities were quantified. Cholinesterase activity was determined in the presence of acetylthiocholine iodide. Both hydrolases were also detected by native poliacrylamide gel electrophoresis, and preliminary characterized by inhibition assays. The possible biological roles of these enzymes in cestodes are discussed.
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