Electrochemical nanobiosensor based on reduced graphene oxide and gold nanoparticles for ultrasensitive detection of microRNA-128
Authors
Mohammadnejad, Javad; Yazdian, Fatemeh; Pourmadadi, Mehrab; Omidi, Meisam; Mirshafiei, Mojdeh; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/57874DOI: https://doi.org/10.1016/j.intimp.2023.109960
ISSN: 1567-5769
Date
2023-03-08Affiliation
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaFunders
Comunidad de Madrid
Bibliographic citation
International Immunopharmacology, 2023, v. 117, n. 109960, p. 1-9
Keywords
Electrochemical nanobiosensors
Methylene blue
HexacyanoferratemicroRNA: Leukemia Real sample
Project
Línea de Actuación de Excelencia del Profesorado Universitario Permanente, del Convenio Plurianual entre la Comunidad de Madrid y la UAH, EPU-INV/2020/012
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
© The authors
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Access rights
info:eu-repo/semantics/openAccess
Abstract
Acute lymphoblastic leukemia (ALL) is one of the most prevalent cancers in children and microRNA-128 is amongst the most useful biomarkers not only for diagnosis of ALL, but also for discriminating ALL from acute myeloid leukemia (AML). In this study, a novel electrochemical nanobiosensor based on reduced graphene oxide (RGO) and gold nanoparticles (AuNPs) has been fabricated to detect miRNA-128. Cyclic Voltametery (CV), Square Wave Voltametery (SWV) and Electrochemical Impedance Spectroscopy (EIS) have been applied to characterize the nanobiosensor. Hexacyanoferrate as a label-free and methylene blue as a labeling material were used in the design of the nanobiosensors. It was found that the modified electrode has excellent selectivity and sensitivity to miR-128, with a limit of detection of 0.08761 fM in label-free and 0.00956 fM in labeling assay. Additionally, the examination of real serum samples of ALL and AML patients and control cases confirms that the designed nanobiosensor has the potential to detect and discriminate these two cancers and the control samples.
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