A high magnesium concentration in citrate dialysate prevents oxidative stress and damage in human monocytes in vitro
Authors
Vida Rueda, María del Carmen; Carracedo Añón, Julia María; Sequera Ortiz, Patricia de; Bodega Magro, Guillermo; Pérez, Rafael; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/51251DOI: 10.1093/ckj/sfaa131
ISSN: 2048-8505
Date
2021-05-06Affiliation
Universidad de Alcalá. Departamento de Biomedicina y Biotecnología; Universidad de Alcalá. Departamento de Biología de SistemasFunders
Instituto de Salud Carlos III
Sociedad Española de Nefrología
Universidad Complutense de Madrid
Universidad de Alcalá
Fondo Europeo de Desarrollo Regional-FEDER
Bibliographic citation
Vida, C. et al., 2021. A high magnesium concentration in citrate dialysate prevents oxidative stress and damage in human monocytes in vitro. Clinical Kidney Journal, 14(5), pp.1403-1411.
Keywords
Acetate
Chronic kidney disease
Citrate
Dialysate
Haemodialysis
Inflammation
Magnesium
Monocytes
Oxidative stress
Project
info:eu-repo/grantAgreement/ISCIII//PI17%2F01029/ES/
info:eu-repo/grantAgreement/ISCIII//PI19%2F00240/ES/
info:eu-repo/grantAgreement/UCM//PR41%2F17-20964/ES/
info:eu-repo/grantAgreement/UAH//UAHGP2018-4/ES/
info:eu-repo/grantAgreement/UAH//CCG2018%2FBIO-010/ES/
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Publisher's version
https://doi.org/10.1093/ckj/sfaa131Rights
Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
© 2020 The Author(s). Published by Oxford University Press on behalf of ERA-EDTA
Access rights
info:eu-repo/semantics/openAccess
Abstract
Background. The use of dialysis fluids (DFs) during haemodialysis has been associated with increased oxidative stress and
reduced serum magnesium (Mg) levels, contributing to chronic inflammation. Since the role of Mg in modulating immune
function and reducing oxidative stress has been demonstrated, the aim of this study was to characterize in vitro whether
increasing the Mg concentration in DFs could protect immune cells from oxidative stress and damage.
Methods. The effect of citrate [citrate dialysis fluid (CDF), 1 mM] or acetate [acetate dialysis fluid (ADF), 3 mM] dialysates with
low (0.5 mM; routinely used) or high (1 mM, 1.25 mM and 2 mM) Mg concentrations was assessed in THP-1 human
monocytes. The levels of reactive oxygen species (ROS), malondialdehyde (MDA) and oxidized/reduced (GSSG/GSH)
glutathione were quantified under basal and inflammatory conditions (stimulation with lipopolysaccharide, LPS).
Results. The increase of Mg in CDF resulted in a significant reduction of ROS production under basal and inflammatory conditions
(extremely marked in 2 mM Mg; P< 0.001). These effects were not observed in ADF. Interestingly, in a dose-dependent manner,
high Mg doses in CDF reduced oxidative stress in monocytes under both basal and inflammatory conditions. In fact, 2 mM Mg
significantly decreased the levels of GSH, GSSG and MDA and the GSSG/GSH ratio in relation to 0.5 mM Mg.
Conclusions. CDF produces lower oxidative stress than ADF. The increase of Mg content in DFs, especially in CDF, could have
a positive and protective effect in reducing oxidative stress and damage in immune cells, especially under inflammatory
conditions.
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