Plum (Prunus Domestica L.) by-product as a new and cheap source of bioactive peptides: extraction method and peptides characterization
IdentifiersPermanent link (URI): http://hdl.handle.net/10017/48507
AffiliationUniversidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química
Journal of Functional Foods, 2014, v. 11, p. 428-437
Ministerio de Economía y Competitividad (AGL2012-36362) Universidad de Alcalá (CCG2013/EXP-028) Comunidad de Madrid y el programa FEDER (S2009/AGR-1464 , ANALISYC-II).
Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
Fruit processing produces a great amount of wastes. Stone of fruits such as plum (Prunus Domestica L.) are part of these residues. Plum stones contain a seed with a high content in proteins and lipids that are mostly underused and undervalued. This work proposes to extract proteins from this by-product, to evaluate their potential as bioactive peptide source, and to identify these peptides using RP-HPLC-MS/MS. A method for the extraction of plum seed proteins using high intensity focused ultrasounds has been developed. Extracted proteins have been digested using Alcalase, Thermolysin, Flavourzyme, and Protease P enzymes. Alcalase was the enzyme yielding the extracts with the highest ABTS radical scavenging capacity and lipid peroxidation inhibition capacity. It also showed a high capacity to scavenge hydroxyl radicals and to inhibit ACE enzyme. Analysis of the Alcalase extract by RP-HPLC-ESI-Q-TOF enabled the identification of short chain and highly hydrophobic peptides. (C) 2014 Elsevier Ltd. All rights reserved.
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