Novel strategy for the revalorization of olive (Olea europaea) residues based on the extraction of bioactive peptides
Identifiers
Permanent link (URI): http://hdl.handle.net/10017/48228DOI: 10.1016/j.foodchem.2014.06.090
ISSN: 0308-8146
Date
2015-01-15Affiliation
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaBibliographic citation
Food Chemistry, 2015, v. 167, p. 272-280
Keywords
Olive seeds
Bioactive peptides
Antioxidant capacity
Antihypertensive capacity
Mass spectrometry
Project
info:eu-repo/grantAgreement/MICINN//CTQ2009-11252/ES/NUEVA ESTRATEGIA BASADA EN EL USO DE PERFILES PROTEICOS PARA LA CARACTERIZACIÓN DE ACEITUNAS Y ACEITE DE OLIVA
info:eu-repo/grantAgreement/MINECO//AGL2012-36362/ES/VALORIZACION DE RESIDUOS SOLIDOS VEGETALES PROCEDENTES DE LA INDUSTRIA AGROALIMENTARIA
info:eu-repo/grantAgreement/CAM//S2009/AGR-1464/ES/METODOLOGÍAS ANALÍTICAS INNOVADORAS PARA EL CONTROL DE LA CALIDAD Y LA SEGURIDAD DE LOS ALIMENTOS
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
© Elsevier
Access rights
info:eu-repo/semantics/openAccess
Abstract
This work proposes a new strategy for the revalorization of residual materials from table-olive and olive oil production based on the extraction of bioactive peptides. Enzymatic hydrolysates of olive seed protein isolate were prepared by treatment with five different proteases: Alcalase, Thermolysin, Neutrase, Flavourzyme and PTN. Although all hydrolysates presented antioxidant properties, Alcalase was the enzyme that yielded the hydrolysate with the highest antioxidant capacity. All hydrolysates showed antihypertensive capacity, obtaining IC50 values from 29 to 350 mu g/ml. Thermolysin was the enzyme which yielded the hydrolysate with the highest ACE-inhibitory capacity. Hydrolysates were fractionated by ultrafiltration showing a high concentration of short chain peptides, which exhibited significantly higher antioxidant and antihypertensive capacities than fractions with higher molecular weights. Peptides in most active fractions were identified by LC-MS/MS, observing homologies with other recognized antioxidant and antihypertensive peptides. Finally, their antioxidant and antihypertensive capacities were evaluated after in vitro gastrointestinal digestion. (C) 2014 Elsevier Ltd. All rights reserved.
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