HPLC-Q-TOF-MS identification of antioxidant and antihypertensive peptides recovered from cherry (Prunus Cerasus L.) subproducts
Autores
García López, María Concepción; Endermann, J; González García, Estefanía; Marina Alegre, María LuisaIdentificadores
Enlace permanente (URI): http://hdl.handle.net/10017/48207DOI: 10.1021/jf505037p
ISSN: 0021-8561
Fecha de publicación
2015-02-11Filiación
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaCita bibliográfica
Journal of Agricultural and Food Chemistry, 2015, v. 63, n. 5, p. 1514-1520
Palabras clave
cherry
seed proteins
peptide
antihypertensive
antioxidant
MS identification
Proyectos
Universidad de Alcalá (CCG2013/EXP-028)
Comunidad de Madrid y financiación europea del programa FEDER (S2009/AGR-1464, ANALISYC-II)
info:eu-repo/grantAgreement/MINECO//AGL2012-36362/ES/VALORIZACION DE RESIDUOS SOLIDOS VEGETALES PROCEDENTES DE LA INDUSTRIA AGROALIMENTARIA/
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/publishedVersion
Derechos
Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
© ACS
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
The processing of fruits, such as cherries, is characterized by generating a lot of waste material such as fruit stones, skins, etc. To contribute to environmental sustainability, it is necessary to recover these residues. Cherry stones contain seeds with a significant amount of proteins that are underused and undervalued. The aim of this work was to extract cherry seed proteins, to evaluate the presence of bioactive peptides, and to identify them by mass spectrometry. The digestion of cherry seed proteins was optimized, and three different enzymes were employed: Alcalase, Thermolysin, and Flavourzyme. Peptide extracts obtained by the digestion of the cherry seed protein isolate with Alcalase and Thermolysin yielded the highest antioxidant and antihypertensive capacities. Ultrafiltration of hydrolysates allowed obtaining fractions with high antioxidant and antihypertensive capabilities. HPLC-Q-TOF-MS together with bioinformatics tools enabled one to identify peptides in these fractions.
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