HPLC-Q-TOF-MS identification of antioxidant and antihypertensive peptides recovered from cherry (Prunus Cerasus L.) subproducts
Authors
García López, María Concepción; Endermann, J; González García, Estefanía; Marina Alegre, María LuisaIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/48207DOI: 10.1021/jf505037p
ISSN: 0021-8561
Date
2015-02-11Affiliation
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaBibliographic citation
Journal of Agricultural and Food Chemistry, 2015, v. 63, n. 5, p. 1514-1520
Keywords
cherry
seed proteins
peptide
antihypertensive
antioxidant
MS identification
Project
Universidad de Alcalá (CCG2013/EXP-028)
Comunidad de Madrid y financiación europea del programa FEDER (S2009/AGR-1464, ANALISYC-II)
info:eu-repo/grantAgreement/MINECO//AGL2012-36362/ES/VALORIZACION DE RESIDUOS SOLIDOS VEGETALES PROCEDENTES DE LA INDUSTRIA AGROALIMENTARIA/
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
© ACS
Access rights
info:eu-repo/semantics/openAccess
Abstract
The processing of fruits, such as cherries, is characterized by generating a lot of waste material such as fruit stones, skins, etc. To contribute to environmental sustainability, it is necessary to recover these residues. Cherry stones contain seeds with a significant amount of proteins that are underused and undervalued. The aim of this work was to extract cherry seed proteins, to evaluate the presence of bioactive peptides, and to identify them by mass spectrometry. The digestion of cherry seed proteins was optimized, and three different enzymes were employed: Alcalase, Thermolysin, and Flavourzyme. Peptide extracts obtained by the digestion of the cherry seed protein isolate with Alcalase and Thermolysin yielded the highest antioxidant and antihypertensive capacities. Ultrafiltration of hydrolysates allowed obtaining fractions with high antioxidant and antihypertensive capabilities. HPLC-Q-TOF-MS together with bioinformatics tools enabled one to identify peptides in these fractions.
Files in this item
Files | Size | Format |
|
---|---|---|---|
HPLC_Garcia_JAgriFoodChem_2015.pdf | 760.8Kb |
|
Files | Size | Format |
|
---|---|---|---|
HPLC_Garcia_JAgriFoodChem_2015.pdf | 760.8Kb |
|
Collections
- QUANING - Artículos [367]