HPLC-Q-TOF-MS identification of antioxidant and antihypertensive peptides recovered from cherry (Prunus Cerasus L.) subproducts

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Authors
García López, María ConcepciónUniversity of Alcalá Author; Endermann, J.; González García, EstefaníaUniversity of Alcalá Author; Marina Alegre, María LuisaUniversity of Alcalá Author
Identifiers
Permanent link (URI): http://hdl.handle.net/10017/48207
DOI: 10.1021/jf505037p
ISSN: 0021-8561
Date
2015-02-11
Academic Departments
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química
Teaching unit
Unidad docente Química Analítica e Ingeniería Quimica
Funders
Universidad de Alcalá
Comunidad de Madrid
Ministerio de Economía y Competitividad
Bibliographic citation
Journal of Agricultural and Food Chemistry, 2015, v. 63, n. 5, p. 1514-1520
Keywords
Cherry
Seed proteins
Peptide
Antihypertensive
Antioxidant
MS identification
Project
info:eu-repo/grantAgreement/CAM//S2009/AGR-1464/ES/Metodologías analíticas innovadoras para el control de la calidad y la seguridad de los alimentos/ANALISYC-II
info:eu-repo/grantAgreement/MINECO//AGL2012-36362/ES/Valorización de residuos sólidos vegetales procedentes de la industria agroalimentaria
info:eu-repo/grantAgreement/UAH//CCG2015%2FEXP-028/ES/Desarrollo de estrategias para mejorar la detección de cocaína por Espectroscopía Raman
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
© ACS
Access rights
info:eu-repo/semantics/openAccess
Abstract
The processing of fruits, such as cherries, is characterized by generating a lot of waste material such as fruit stones, skins, etc. To contribute to environmental sustainability, it is necessary to recover these residues. Cherry stones contain seeds with a significant amount of proteins that are underused and undervalued. The aim of this work was to extract cherry seed proteins, to evaluate the presence of bioactive peptides, and to identify them by mass spectrometry. The digestion of cherry seed proteins was optimized, and three different enzymes were employed: Alcalase, Thermolysin, and Flavourzyme. Peptide extracts obtained by the digestion of the cherry seed protein isolate with Alcalase and Thermolysin yielded the highest antioxidant and antihypertensive capacities. Ultrafiltration of hydrolysates allowed obtaining fractions with high antioxidant and antihypertensive capabilities. HPLC-Q-TOF-MS together with bioinformatics tools enabled one to identify peptides in these fractions.

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