Show simple item record

dc.contributor.authorMarina Alegre, María Luisa 
dc.contributor.authorCastro Puyana, María 
dc.contributor.authorGreño Ocariz, Maider 
dc.date.accessioned2021-05-25T07:13:17Z
dc.date.issued2020-09
dc.identifier.bibliographicCitationMicrochemical Journal, 2020, v. 157, n. 105070, p. 1-8en
dc.identifier.issn0026-265X
dc.identifier.urihttp://hdl.handle.net/10017/48156en
dc.description.abstractTwo new chiral ionic liquids based on the ester of the non-protein amino acid carnitine as cationic counterpart were synthesized, characterized and evaluated as chiral selectors for the enantiomeric separation of homocysteine and cysteine derivatized with 9-fluorenylmethoxycarbonyl chloride by electrokinetic chromatography. The use of both ionic liquids, L-carnitine methyl ester bis(trifluoromethane)sulfonimide and L-carnitine methyl ester L-(+)-lactate, as sole chiral selectors in the separation buffer, did not allow the enantiomeric separation of the studied amino acids. However, the combined use of L-carnitine methyl ester bis(trifluoromethane)sulfonimide ionic liquid with gamma-CD forming a dual system led to the enantiomeric separation of both analytes and showed the existence of a strong synergistic effect. On the contrary, the dual system gamma-CD plus L-carnitine methyl ester L-(+)-lactate did not improve the enantiomeric separations with respect to those obtained using the CD alone. The influence of different experimental variables such as buffer composition and pH, and ionic liquid concentration was investigated. Also, the nature of the anionic moiety was evaluated by comparing the results obtained with both ionic liquids when combined with gamma-CD. The use of 2 mM gamma-CD combined with 5 mM L-carnitine methyl ester bis(trifluoromethane)sulfonimide in a 50 mM phosphate buffer at pH 7.0 enabled to achieve the simultaneous enantiomeric separation of homocysteine and cysteine with high resolution values (> 6.0) in analysis times close to 12 min.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)en
dc.rights© Elsevieren
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en
dc.subjectCarnitine ionic liquidsen
dc.subjectCapillary electrophoresisen
dc.subjectCyclodextrinsen
dc.subjectHomocysteineen
dc.subjectCysteineen
dc.subjectEnantiomersen
dc.titleEnantiomeric separation of homocysteine and cysteine by electrokinetic chromatography using mixtures of gamma-cyclodextrin and carnitine-based ionic liquidsen
dc.typeinfo:eu-repo/semantics/articleen
dc.subject.ecienciaQuímicaes_ES
dc.subject.ecienciaChemistryen
dc.contributor.affiliationUniversidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Químicaes_ES
dc.contributor.affiliationUniversidad de Alcalá. Departamento de Química Orgánica y Química Inorgánicaes_ES
dc.date.updated2021-05-25T07:11:34Z
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.identifier.doi10.1016/j.microc.2020.105070en
dc.relation.projectIDinfo:eu-repo/grantAgreement/CAM//S2018%2FBAA-4393/ES/ESTRATEGIAS INTEGRADAS PARA LA MEJORA DE LA CALIDAD, LA SEGURIDAD Y LA FUNCIONALIDAD DE LOS ALIMENTOS: HACIA UNA ALIMENTACIÓN SALUDABLE/AVANSECAL-IIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//CTQ2016-76368-P/ES/NUEVOS NANOADITIVOS PARA SEPARACIÓN QUIRAL POR ELECTROFORESIS CAPILAR: APLICACIÓN A LA DETERMINACIÓN DE BIOMARCADORESes_ES
dc.date.embargoEndDate2022-10-01
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.uxxiAR/0000034327en
dc.identifier.publicationtitleMicrochemical Journalen
dc.identifier.publicationvolume157
dc.identifier.publicationlastpage8
dc.identifier.publicationissue105070
dc.identifier.publicationfirstpage1


Files in this item

Thumbnail

This item appears in the following Collection(s)

Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
Este ítem está sujeto a una licencia Creative Commons.