In vitro antitumor and hypotensive activity of peptides from olive seeds

Authors
Vásquez Villanueva, Romy Angela; Muñoz Moreno, LauraUniversity of Alcalá Author; Carmena Sierra, María JoséUniversity of Alcalá Author; Marina Alegre, María LuisaUniversity of Alcalá Author; García López, María ConcepciónUniversity of Alcalá Author
Identifiers
Permanent link (URI): http://hdl.handle.net/10017/48153
DOI: 10.1016/j.jff.2017.12.062
ISSN: 1756-4646
Date
2018-03
Affiliation
Universidad de Alcalá. Departamento de Biología de Sistemas; Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química
Bibliographic citation
Journal of Functional Foods, 2018, v. 42, p. 177-184
Keywords
Peptide
Olive seeds
Antitumor capacity
Antihypertensive capacity
Project
Ministerio de Economía y Competitividad (AGL2016-79010-R) Comunidad de Madrid y programa FEDER (S2013/ABI-3028 AVANSECAL-CM)
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
© Elsevier
Access rights
info:eu-repo/semantics/openAccess
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Abstract
Peptides with molecular weights below 3 kDa from the hydrolysis of olive seed proteins with Thermolysin (OS-3 kDa) have demonstrated a high in vitro hypotensive effect. This fraction has been further fractionated by semipreparative RP-HPLC to obtain 8 fractions. ACE inhibitor capacity of fractions was evaluated observing the highest capacity in fraction F5. Peptides in fraction F5 were identified by RP-HPLC- and HILIC-ESI-Q-ToF and cytotoxic effect was assessed in different cell lines. Peptide LLPSY, present in this faction, was synthesized and characterized. Despite antihypertensive capacity was not as high as in fraction F5, a significant anti-proliferative capacity on two different cancer cell lines was observed. Additional studies to assess antitumor activity confirmed that this peptide showed capability to increase the adhesion capacity of tumor cells, to decrease the migration capacity of cancer cells, and to arrest cell cycle on S phase.
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