Factors affecting interactions between sulphonate-terminated dendrimers and proteins: A three case study
Authors
González García, Estefanía; Maly, Marek; Mata de la Mata, Francisco Javier de la; Gómez Ramírez, Rafael; Marina Alegre, María Luisa; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/48152DOI: 10.1016/j.colsurfb.2016.10.020
ISSN: 0927-7765
Date
2017-01-01Affiliation
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química; Universidad de Alcalá. Departamento de Química Orgánica y Química InorgánicaBibliographic citation
Colloids and Surfaces B: Biointerfaces, 2017, v. 149, p. 196-205
Keywords
Sulphonate-terminated carbosilane dendrimers
Protein-dendrimer interaction
Fluorescence
Quenching
Computer modeling
Molecular dynamics
Project
info:eu-repo/grantAgreement/GARC//GA13-06989S/ES/Materials engineering towards Innovative Graetzel solar cells
info:eu-repo/grantAgreement/MINECO//AGL2012-36362/ES/VALORIZACION DE RESIDUOS SOLIDOS VEGETALES PROCEDENTES DE LA INDUSTRIA AGROALIMENTARIA/
info:eu-repo/grantAgreement/MINECO//CTQ-2014-54004-P//ES
info:eu-repo/grantAgreement/CAM//S2011%2FBMD-2351//ES
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
© Elsevier
Access rights
info:eu-repo/semantics/openAccess
Abstract
This work proposes a deep study on the interactions between sulphonate-terminated carbosilane dendrimers and proteins. Three different proteins with different molecular weights and isoelectric points were employed and different pHs, dendrimer concentrations and generations were tested. Variations in fluorescence intensity and emission wavelength were used as protein-dendrimer interaction probes. Interaction between dendrimers and proteins greatly depended on the protein itself and pH. Other important issues were the dendrimer concentration and generation. Protein-dendrimer interactions were favored under acidic working conditions when proteins were positively charged. Moreover, in general, high dendrimer generations promoted these interactions. Modeling of protein-dendrimer interactions allowed to understand the different behaviors observed for every protein. (C) 2016 Elsevier B.V. All rights reserved.
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