Enantiomeric determination of econazole and sulconazole by electrokinetic chromatography using hydroxypropyl-b-cyclodextrin combined with ionic liquids based on L-lysine and Ly-glutamic acid
Identifiers
Permanent link (URI): http://hdl.handle.net/10017/47167DOI: 10.1016/j.chroma.2020.461085
ISSN: 00219673
Date
2020-06-21Embargo end date
2022-06-21Affiliation
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaBibliographic citation
Journal of Chromatography A, 2020, v. 1621, n. 461085, p. 1-8
Keywords
Electrokinetic chromatography
Amino acid based chiral ionic liquids
Cyclodextrin
Enantioseparation
Econazole
Sulconazole
Project
CTQ2016-76368-P (Ministerio de Economía y Competitividad de España)
S2018/BAA-4393 (AVANSECAL-II-CM)
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
© Elsevier
Access rights
info:eu-repo/semantics/openAccess
Abstract
Two analytical methodologies based on the combined use of hydroxypropyl-beta-cyclodextrin and two different amino acid-based chiral ionic liquids (tetrabutylammonium-L-lysine or tetrabutylammonium-L-glutamic acid) in electrokinetic chromatography were developed in this work to perform the enantios-elective determination of econazole and sulconazole in pharmaceutical formulations. The influence of different experimental variables such as buffer concentration, applied voltage, nature and concentration of the ionic liquid, temperature and injection time, on the enantiomeric separation was investigated. The combination of hydroxypropyl-beta-cyclodextrin and tetrabutylammonium-L-lysine under the optimized conditions enabled to achieve the enantiomeric determination of both drugs with high enantiomeric resolution (3.5 for econazole and 2.4 for sulconazole). The analytical characteristics of the developed methodologies were evaluated in terms of linearity, precision, LOD, LOQ and recovery showing good performance for the determination of both drugs which were successfully quantitated in pharmaceutical formulations. This work reports the first analytical methodology enabling the enantiomeric determination of sulconazole in pharmaceutical formulations.
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