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dc.contributor.authorGonzález García, Estefanía 
dc.contributor.authorGarcía López, María Concepción 
dc.contributor.authorMarina Alegre, María Luisa 
dc.date.accessioned2020-10-29T08:42:50Z
dc.date.available2020-10-29T08:42:50Z
dc.date.issued2018-03-09
dc.identifier.bibliographicCitationJournal of Chromatography A., 2018, v. 1540, p. 47-54en
dc.identifier.issn0021-967
dc.identifier.urihttp://hdl.handle.net/10017/44847en
dc.description.abstractPrunus genus fruit seeds are sources of highly angiotensin-l-converting enzyme (ACE)-inhibitory peptides. The presence of peptides IYSPH, IYTPH, IFSPR, and VAIP seems to be related to this activity but no previous work has demonstrated the direct relationship between the concentration of these peptides and the antihypertensive activity of hydrolysates. This work describes the development of a method for the quantification of these peptides in Prunus seeds hydrolysates based on capillary liquid chromatography-IT-MS/MS. The analytical characteristics of the method were evaluated through the study of the linearity, LOD, LOQ presence of matrix interferences, precision, and recovery. The developed methodology was applied to the determination of the four peptides in seed hydrolysates from different Prunus genus fruits: peaches (7 varieties), plums (2 varieties), nectarines (3 varieties), apricots (2 varieties), cherry, and paraguayo. Peaches and plums seed hydrolysates yielded the highest concentrations of these peptides while paraguayo one showed the lowest concentrations. A high correlation between peptides concentrations was demonstrated suggesting that the four peptides could be released from the same seed proteins.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)en
dc.rights© Elsevieren
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en
dc.subjectACE inhibitory peptidesen
dc.subjectQuantificationen
dc.subjectCapillary-HPLCen
dc.subjectIon trap mass spectrometryen
dc.subjectPrunus seedsen
dc.titleCapillary liquid-chromatography-ion trap-mass spectrometry methodology for the simultaneous quantification of four angiotensin-converting enzyme-inhibitory peptides in Prunus seed hydrolysatesen
dc.typeinfo:eu-repo/semantics/articleen
dc.subject.ecienciaQuímicaes_ES
dc.subject.ecienciaChemistryen
dc.contributor.affiliationUniversidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química
dc.date.updated2020-10-29T08:42:14Z
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.identifier.doi10.1016/j.chroma.2018.02.003en
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//AGL2016-79010-R/ES/DESARROLLO DE METODOS SOSTENIBLES PARA EL APROVECHAMIENTO DE SUBPRODUCTOS DE LA INDUSTRIA ALIMENTARIA CON ELEVADO CONTENIDO PROTEICOes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/CAM//S2013%2FABI-3028/ES/Estrategias avanzadas para la mejora y el control de la calidad y la seguridad de los alimentos/AVANSECAL-CMes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.uxxiAR/0000027693en
dc.identifier.publicationtitleJournal of Chromatography A.en
dc.identifier.publicationvolume1540
dc.identifier.publicationlastpage54
dc.identifier.publicationfirstpage47


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