Time-series proteomic study of the response of HK-2 cells to hyperglycemic, hypoxic diabetic-like milieu
Authors
Valdés Tabernero, Alberto; García Pastor, Coral; Castro Puyana, María; Lucio Cazaña, Francisco Javier de; Marina Alegre, María LuisaIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/44732DOI: 10.1371/journal.pone.0235118
ISSN: 1932-6203
Date
2020-06-24Affiliation
Universidad de Alcalá. Departamento de Biología de Sistemas; Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaBibliographic citation
PLoS ONE, 2020, v. 15, n. 6, p. E0235118
Project
Ministerio de Economía y Competitividad de España
(CTQ2016-76368-P, SAF2014-53218-R, FJCI-2016-30741, y RYC2013-12688), Comunidad de Madrid y los programas europeos FSE y FEDER (S2018 / BAA-4393 - AVANSECAL-II-CM y B2017 / BMD3686)
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
Public Library Science
Access rights
info:eu-repo/semantics/openAccess
Abstract
During diabetes, renal proximal tubular cells (PTC) are exposed to a combination of high glucose and hypoxic conditions, which plays a relevant role in the development of diabetic kidney disease (DKD). In this work, a time-series proteomic study was performed to analyse the effect of a diabetic-like microenvironment induced changes on HK-2 cells, a human cell line derived from normal proximal tubular epithelial cells. Cells simultaneously
exposed to high glucose (25 mM) and hypoxia (1% O-2) were compared to cells in control conditions
for up to 48 h. Diabetic conditions increased the percentage of death cells after 24 and 48 h,
but no differences in the protein/cell ratio were found. The relative protein
quantification using dimethyl-labeling and UHPLC-MS/MS analysis allowed the identification of 317, 296 and 259
proteins at 5, 24 and 48 h, respectively. The combination of statistical and time expression profile analyses indicated an
increased expression of proteins involved in glycolysis, and a decrease of cytoskeletal-related
proteins. The exposure of HK-2 cells to high glucose and hypoxia reproduces some of the effects of diabetes on PTC and,
with the limitations inherent toin vitrostudies, propose new mechanisms
and targets to be considered in the management of DKD.
Files in this item
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Time-series_Valdes_PlosOne_2020.pdf | 1.548Mb |
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Time-series_Valdes_PlosOne_2020.pdf | 1.548Mb |
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