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dc.contributor.authorMarina Alegre, María Luisa 
dc.contributor.authorGarcía López, María Concepción 
dc.contributor.authorGonzález García, Estefanía 
dc.contributor.authorMata de la Mata, Francisco Javier de la 
dc.contributor.authorSánchez-Nieves Fernández, Javier 
dc.date.accessioned2020-10-14T07:20:42Z
dc.date.issued2020-02
dc.identifier.bibliographicCitationColloids and Surfaces B: Biointerfaces, 2020, v. 186, n. 110746, p. 1-8en
dc.identifier.issn0927-7765
dc.identifier.urihttp://hdl.handle.net/10017/44568en
dc.description.abstractProtein sample preparation is the bottleneck in the analysis of proteins. The aim of this work is to evaluate the feasibility of carbosilane dendrimers functionalized with cationic groups to make easier this step. Anionic carbosilane dendrimers (sulphonate- and carboxylate-terminated) have already demonstrated their interaction with proteins and their potential in protein sample preparation. In this work, interactions between positively charged carbosilane dendrimers and different model proteins were studied when working under different pH conditions, dendrimer concentrations, and dendrimer generations. Amino- and trimethylammonium-terminated carbosilane dendrimers presented, in some cases, weak interactions with proteins. Unlike them, carbosilane dendrimers with terminal dimethylamino groups could interact, in many cases, with proteins and these interactions were affected by the pH, the dendrimer concentration, and the dendrimer generation. Moreover, dendrimer precipitation was observed at all pHs, although just second and fourth generation (2 G and 4 G) dendrimers resulted in the formation of complexes with proteins. Under experimental conditions promoting dendrimer-protein interactions, 2 G dimethylamino-terminated dendrimers were proposed as an alternative to other methods used in analytical chemistry or analysis in which an organic solvent or a resin are required to enrich/purify proteins in a complex sample.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)en
dc.rights© Elsevieren
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectCarbosilane dendrimeren
dc.subjectCationic dendrimeren
dc.subjectInteractionen
dc.subjectProtein enrichmenten
dc.subjectProtein purificationen
dc.titleFeasibility of cationic carbosilane dendrimers for sustainable protein sample preparationen
dc.typeinfo:eu-repo/semantics/articleen
dc.subject.ecienciaQuímicaes_ES
dc.subject.ecienciaChemistryen
dc.contributor.affiliationUniversidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química
dc.contributor.affiliationUniversidad de Alcalá. Departamento de Química Orgánica y Química Inorgánica
dc.date.updated2020-10-14T07:19:41Z
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.identifier.doi10.1016/j.colsurfb.2019.110746en
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//AGL2016-79010-R/ES/DESARROLLO DE METODOS SOSTENIBLES PARA EL APROVECHAMIENTO DE SUBPRODUCTOS DE LA INDUSTRIA ALIMENTARIA CON ELEVADO CONTENIDO PROTEICOes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/CAM//S2018%2FBAA-4393/ES/ESTRATEGIAS INTEGRADAS PARA LA MEJORA DE LA CALIDAD, LA SEGURIDAD Y LA FUNCIONALIDAD DE LOS ALIMENTOS: HACIA UNA ALIMENTACIÓN SALUDABLE/AVANSECAL-IIes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/CAM//CTQ2017-86224-P/ES/DENDRONIZACIÓN COMO HERRAMIENTA SINTÉTICA DE OBTENCIÓN DE NANOSISTEMAS DENDRÍTICOS ESPECÍFICAMENTE DISEÑADOS PARA SU USO EN APLICACIONES BIOMÉDICASES_es
dc.date.embargoEndDate2022-03-01
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.uxxiAR/0000033905en
dc.identifier.publicationtitleColloids and Surfaces B: Biointerfacesen
dc.identifier.publicationvolume186
dc.identifier.publicationlastpage8
dc.identifier.publicationissue110746
dc.identifier.publicationfirstpage1


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