Feasibility of cationic carbosilane dendrimers for sustainable protein sample preparation
AuthorsMarina Alegre, María Luisa; García López, María Concepción; González García, Estefanía; Mata de la Mata, Francisco Javier de la; Sánchez-Nieves Fernández, Javier
IdentifiersPermanent link (URI): http://hdl.handle.net/10017/44568
Embargo end date2022-03-01
AffiliationUniversidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química; Universidad de Alcalá. Departamento de Química Orgánica y Química Inorgánica
Colloids and Surfaces B: Biointerfaces, 2020, v. 186, n. 110746, p. 1-8
AGL2016-79010-R y CTQ2017-86224-P (Ministerio de Economía y Competitividad de España, Comunidad de Madrid) y financiación europea de los programas FSE y FEDER (S2018 / BAA-4393, AVANSECAL-II-CM) .
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Protein sample preparation is the bottleneck in the analysis of proteins. The aim of this work is to evaluate the feasibility of carbosilane dendrimers functionalized with cationic groups to make easier this step. Anionic carbosilane dendrimers (sulphonate- and carboxylate-terminated) have already demonstrated their interaction with proteins and their potential in protein sample preparation. In this work, interactions between positively charged carbosilane dendrimers and different model proteins were studied when working under different pH conditions, dendrimer concentrations, and dendrimer generations. Amino- and trimethylammonium-terminated carbosilane dendrimers presented, in some cases, weak interactions with proteins. Unlike them, carbosilane dendrimers with terminal dimethylamino groups could interact, in many cases, with proteins and these interactions were affected by the pH, the dendrimer concentration, and the dendrimer generation. Moreover, dendrimer precipitation was observed at all pHs, although just second and fourth generation (2 G and 4 G) dendrimers resulted in the formation of complexes with proteins. Under experimental conditions promoting dendrimer-protein interactions, 2 G dimethylamino-terminated dendrimers were proposed as an alternative to other methods used in analytical chemistry or analysis in which an organic solvent or a resin are required to enrich/purify proteins in a complex sample.
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